Sequences from confirmation amplicon


Confirmation sequences for line SALK_012172c and hit At4g31580 are shown.
Line ID SALK_012172c
Locus At4g31580 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr:
Primers used for PCR 11pt - LBb3, show primer details
   
Sequence Name 43-K115373-SALK_012172C-11pt
GenBank Accession LN485356 [GenBank]
Sequencing primer gene specific primer, 11pt: 5'-TTGATTGGATTGATTCTTACCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K115373-SALK_012172C-11pt
TCAGTTCACGCTCAGTAACTCGCGGGTCCAATTTCCGACGTACACACGTGACATTACTTC
TGTAACCTGTCAATGGCACAATAAACCAAGGTCAACATAAAGGAATTCTAAATCTTTTCT
AGTAAGACATTCGAAAGGATTTGAATTCATCGAGAAAAAATACAGAGTCACTAGATCCAC
CAAGTGACCCGATCAAATTTGAACAGATCGGATCAACGTTACCACAATTAAGAGTGAGAA
GGAGATAAAGAAACTTGGTTGCGACCTACCTTGTGGGAAGAGCTGAAAGCACTCAAGGGG
CAAAAAGAATGGCTTTTTCTGTGTTTTGCTTTGTGAAGAAATTAGGGTTTCTGAATCTGA
AATTTGATGAAATACTTTCCAAAGGGTTTTGTAAGCGCGTTAACAAAATTTAGTAAATAT
ACAATTTGGGCCTATTTATTGGGCTTAAAAGTAGCCTTTTCATGCCTTTTTTCAATAATA
TGTTAGTGTCGGTGATATTTATTTTTTCTCTGTATAGTTTGTTAAATACTTAAATGTGAT
CATTTCACGTATTTTGCTTTGTATTTCTGAAAATAATGAATAGCAGAATAGGGAAAAAGA
AAAATAATAGAACTTCAAGTCTCTCTACAACTGACGCTTATTGTGGTGTAAACAAATTGA
CGCTNAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTT
TTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGC
AGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGT
   
Sequence Name 41-K115372-SALK_012172C-R210
GenBank Accession LN485314 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K115372-SALK_012172C-R210
CATTTGTTTACCCACAATAAGCGTCAGTTGTAGAGAGACTTGAAGTTCTATTATTTTTCT
TTTTCCCTATTCTGCTATTCATTATTTTCAGAAATACAAAGCAAAATACGTGAAATGATC
ACATTTAAGTATTTAACAAACTATACAGAGAAAAAATAAATATCACCGACACTAACATAT
TATTGAAAAAAGGCATGAAAAGGCTACTTTTAAGCCCAATAAATAGGCCCAAATTGTATA
TTTACTAAATTTTGTTAACGCGCTTACAAAACCCTTTGGAAAGTATTTCATCAAATTTCA
GATTCAGAAACCCTAATTTCTTCACAAAGCAAAACACAGAAAAAGCCATTCTTTTTGCCC
CTTGAGTGCTTTCAGCTCTTCCCACAAGGTAGGTCGCAACCAAGTTTCTTTATCTCCTTC
TCACTCTTAATTGTGGTAACGTTGATCCGATCTGTTCAAATTTGATCGGGTCACTTGGTG
GATCTAGTGACTCTGTATTTTTTCTCGATGAATTCAAATCCTTTCGAATGTCTTACTAGA
AAAGATTTAGAATTCCTTTATGTTGACCTTGGTTTATTGTGCCATTGACAGGTTACAGAA
GTAATGTCACGTGTGTACGTCGGAAATTTGGACCCGCGAGTTACTGAGCGTGAACTTGAG
GATGAGTTCCGTGCCTTTGGAGTTGTCAGGAGGTAAG

Last Updated on 06.12.2018 16:11