Sequences from confirmation amplicon


Confirmation sequences for line SALK_021474c and hit At3g45000 are shown.
Line ID SALK_021474c
Locus At3g45000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:16460340
Primers used for PCR 17oz - LBb3, show primer details
   
Sequence Name 17-K115143-SALK_021474C-17oz
GenBank Accession LN484752 [GenBank]
Sequencing primer gene specific primer, 17oz: 5'-TAAAGCGGAAGCAAAACATTTAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K115143-SALK_021474C-17oz
GGGCTGCCTTATGCCTATCTCCAATTTGGGCTTTCATTATTGGTGAAACGAAGAAGCCCA
AATGTTGACCTATTTTATAAACACTGAAAACATTCGTACTCAAAAGTGAAATTTTAAAAC
TTTGAGGGCTCTTTGTCAATTGTTCATGGATTCATCTTCTCTCTTTGAAGTTTGAACTTT
GAACTGAAGAAACAGAATTTGAAATGATACAAAGTTCAAAGCTTTTGGAATTGCAAAATC
AAAAGTTTCAAGAAGTAAGAAAGAAATGAAGAGAGTGATCAGATTCTTTAAGCGGAAGTC
TGATCCTAAGCAAAAGCTTCGAGATTGGCAACGAAAGCTACGATAAGATGTCCGTGACAT
TGAACGACAAATTCGAGGTTCTTGTTGCTTTTTTCTCTTGTATTTGATCAGATTCTTAGG
AAAGTTTCGAAATTTGAGGAATTTAGTTAGTTATATGATGATTTATATTGATGGGTTTAG
GGTTTTAGATTAAAGATCCAATTTTTGAGGACAAATTGACGCTTAGACAACTTAATAACA
CATTGCGGACGTTTTTAATGTACTGAGGTGGTTTTTCTTTTCTCTTGTGAAACGGGGAAC
AGCTGATTGCTCTTCC
   
Sequence Name 01-K115142-SALK_021474C-R210
GenBank Accession LN484288 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K115142-SALK_021474C-R210
GTCATTTGTCCTCAGAATTGGATCTTTAATCTAAAACCGTAAACCCATCAATATGAATCA
TCATATAACTAACTAAATTCCTCAAATTTCGAAACTTTCCTAAGAATCTGATCAAATACC
AGAGAGAAAAGCAACAAGAACCTCGAATTTGTCGTTCAATGTCACGGACATCTAATCGTA
GCTTTCGTTGCCAATCTCGAAGCTGTTGCTTAGGATCAGACTTCCGCTTAAAGAATCTGA
TCACTCTCTTCATTTCTTTCTTACTTCTTGAAACTTTTGATTTTGCAATTCCGAAAGCTT
TGAACTTTGTATCACTTCAAATTCGGTTTCTTCAGTTCAAAGTTCAAACTTCAGAGAGAG
AAGATGAATCCATGAACAATTGACAAAGAGCCCTCAGAGTTTTAAAATTTCACTTTTGAG
TACGAATGTTTTCAGTGTTTATAAAAGAGGTCAACATTTGGGCTTCGTCGGTTCACCGGT
TATGAATGCCCAGATTT

Last Updated on 06.12.2018 16:11