Sequences from confirmation amplicon


Confirmation sequences for line SALK_023726c and hit At5g45430 are shown.
Line ID SALK_023726c
Locus At5g45430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:18411360
Primers used for PCR 22kn - LBb3, show primer details
   
Sequence Name 53-K113870-SALK_023726C-22kn
GenBank Accession LN485585 [GenBank]
Sequencing primer gene specific primer, 22kn: 5'-TTGATGAGGGCTCGTAACTTAGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K113870-SALK_023726C-22kn
ACGGCGATTTTCTAGAGTAGGGATTGGAAGGCTGTGTAGGTCTGAGGAACATGTCACCAC
TTTCTCCCACCCACCCTGCCTTCATCGCCGGTTGTTGTTGCCTTTGACTTTGAGTCCATC
TTCCTCCAATTGTTGCATTTGCCAGTTTATTTGCAGTCTCTGATGCTCCACGGGAAAAGA
CCCAGTTCTTTTCCATAGATGCTGCAACTGCAGAGTTTGAATTCATTCATTTAGATGATT
AAAAAAAAATCTTTGTGAAGGCAAAAGATGTGAATTTTACTTACGTGGACTCTTCCTTCC
CGGTGGTCTATACTTTGAATCTTTAACGTTATAACTTCTCACAGGTTTGTTGGAACAAAT
TGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTT
CTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGT
TGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTT
CCGA
   
Sequence Name 61-K113869-SALK_023726C-R210
GenBank Accession LN485767 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K113869-SALK_023726C-R210
TTTGTTCCACAACCTGTGAGAAGTTATAACGTTAAAGATTCAAAGTATAGACCACCNGGG
AAGGAAGAGTCCACGTAAGTAAAATTCACATCTTTTGCCTTCACAAAGATTTTTTTTTAA
TCATCTAAATGAATGAATTCAAACTCTGCAGTTGCAGCATCTATGGAAAAGAACTGGGTC
TTTTCCCGTGGAGCATCAGAGACTGCAAATAAACTGGCAAATGCAACAATTGGAGGAAGA
TGGACTCAAAGTCAAAGGCAACAACAACCGGCGATGAAGGCAGGGTGGGTGGGAGAAAGT
GGTGACATGTTCCTCAGACCTACACAGCCTTCCAATCCCTACTCTAGGAAAATCGCCGGT
TAAGCTGAAAGATTCTGTTATGGCGCATCTAAGTTACGAGCCCTCATCA

Last Updated on 06.12.2018 16:11