Sequences from confirmation amplicon


Confirmation sequences for line SALK_026330c and hit At3g14010 are shown.
Line ID SALK_026330c
Locus At3g14010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:4638151
Primers used for PCR 15jd - LBb3, show primer details
   
Sequence Name 21-K113259-SALK_026330C-15jd
GenBank Accession LN484885 [GenBank]
Sequencing primer gene specific primer, 15jd: 5'-GTATAGAGGTCCTCGTCGAAAGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K113259-SALK_026330C-15jd
CTCAACTGATTCATTTCCTACAAGCAGAAACGGATCAGCTGAGAGATATCAAGGACAGAA
AAGACAGGTAACCACACAGCAAAGATTACTCATATATAAGAAACATAACTATCAAAACAA
TATTCTTCCTAATCTTTGAGACATTCACCATGGGCTGTTCCTCACAATATAATATATGCT
TTCACTAAATAGTAGAATTCTAGCTTTAGATATAGAAAGAGAATTGCTTTCCAAAAGGAA
TATACGGAATTTCTAAAGTGCAAGCACAAACATAATCTATTCATTCCCACCATCTCTCAT
CTAAATTGGTCAGCTAAGAAACCGTGATCTGAAAATCTAGCTGACAAATCAAACACATTG
CGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTG
ATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCC
CAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAATCGNGCAAAA
   
Sequence Name 04-K113258w-SALK_026330C-R210
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K113258w-SALK_026330C-R210
ATCACGGTTTCTTAGCTGACAATTTAGATGAGAGATGGTGGGAATGAATAGATTATGTTT
GTGCTTGCACTTTAGAAATTCCGTATATTCCTTTTGGAAAGCAATTCTCTTTCTATATCT
AAAGCTAGAATTCTACTATTTAGTGAAAGCATATATTATATTGTGAGGAACAGCCCATGG
TGAATGTCTCAAAGATTAGGAAGAATATTGTTTTGATAGTTATGTTTCTTATATATGAGT
AATCTTTGCTGTGTGGTTACCTGTCTTTTCTGTCCTTGATATCTCTCAGCTGATCCGTTT
CTGCTTGTAGGAAATGGAATCAGTTTGAGGTGAATAAGTCACTGTTTGGAGTAAAAAGCA
CTTTCGACGAGGAACTCTATA

Last Updated on 06.12.2018 16:11