Sequences from confirmation amplicon


Confirmation sequences for line SALK_029203c and hit At5g02870 are shown.
Line ID SALK_029203c
Locus At5g02870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:657893
Primers used for PCR 09ii - LBb3, show primer details
   
Sequence Name 31-K112623-SALK_029203C-09ii
GenBank Accession LN485147 [GenBank]
Sequencing primer gene specific primer, 09ii: 5'-TAGAAATGTAACGACGATTCCTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K112623-SALK_029203C-09ii
ATTCATACTGTTCTAGCTTTCTCCGCATCGTCGTAAGCACCGATCTGCTTCAATACCTTG
ATCGCAGCTGATGTCTTCTCCACAGCTTCAGCTGAGTCGCTAACAACAAGAGGCATCTCA
GGAACATTCTCGATCTTGTGACCACGAGCCATCACAAGAGCTGGAACAGCAGTAGCAGCG
ATTGCAGAAACAATCGCGTGCCTCTTCATGTTGACATTGACACGACGGTGCCAGCGTCTC
CAGATCTTAGTCGGAGCAAACATACGACCACCACGACACATGTTTCCGAACGCTGCTTGA
CCGGCACGGTGAGTTCCACCACCAGGAACACGAGGGATACGTGACACGGCTCTTCCGGTT
CCCCAGGACTCGGCGGAGGTTTGGTGACCGGCCTTTTTGGAGACTGCGTAAGGCTGACGG
CTGTTGTTGGAGATTTGGGCGTGGACGAAGTTGACAATGTCAGGTCGAACTGGAGCAGTC
ATGACGTCTGGTAAAGTGACGGTGGTGGATTGATCGGTAAACGTCATTGAAAAGAATATA
TTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAAT
GTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCG
CCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAAT
CCTGTTTGATGGTGGTTCCG
   
Sequence Name 07-K112622-SALK_029203C-R210
GenBank Accession LN484461 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K112622-SALK_029203C-R210
CATTTGTTACACCACAATATATTCTTTTCATGACGTTTACCGATCAATCCACCACCGTCA
CTTTACCAGACGTCATGACTGCTCCAGTTCGACCTGACATTGTCAACTTCGTCCACGCCC
AAATCTCCAACAACAGCCGTCAGCCTTACGCAGTCTCCAAAAAGGCCGGTCACCAAACCT
CCGCCGAGTCCTGGGGAACCGGAAGAGCCGTGTCACGTATCCCTCGTGTTCCTGGTGGTG
GAACTCACCGTGCCGGTCAAGCAGCGTTCGGAAACATGTGTCGTGGTGGTCGTATGTTTG
CTCCGACTAAGATCTGGAGACGCTGGCACCGTCGTGTCAATGTCAACATGAAGAGGCACG
CGATTGTTTCTGCAATCGCTGCTACTGCTGTTCCAGCTCTTGTGATGGCTCGTGGTCACA
AGATCGAGAATGTTCCTGAGATGCCTCTTGTTGTTAGCGACTCAGCTGAAGCTGTGGAGA
AGACATCAGCTGCGATCAAGGTATTGAAGCAGATCGGTGCTTACGACGATGCGGAGAAAG
CTAAGAACAGTATTGGAATTCGTCCTGGTAAAGGTAAAATGAGGAATCGTCGTTACATTT
CT
Primers used for PCR 09gq - LBb3, show primer details
   
Sequence Name 86-K112710-SALK_029203C-09gq
GenBank Accession LN486297 [GenBank]
Sequencing primer gene specific primer, 09gq: 5'-CGATTCCTCATTTTACCTTTACCA-3'
Sequence (A. th genome BLAST matches underlined) 
>86-K112710-SALK_029203C-09gq
TCGTAAGCCCGATCTGCTTCATACCTTGATCGCAGCTGATGTCTTCTCCACAGCTTCAGC
TGAGTCGCTAACAACAAGAGGCATCTCAGGAACATTCTCGATCTTGTGACCACGAGCCAT
CACAAGAGCTGGAACAGCAGTAGCAGCGATTGCAGAAACAATCGCGTGCCTCTTCATGTT
GACATTGACACGACGGTGCCAGCGTCTCCAGATCTTAGTCGGAGCAAACATACGACCACC
ACGACACATGTTTCCGAACGCTGCTTGACCGGCACGGTGAGTTCCACCACCAGGAACACG
AGGGATACGTGACACGGCTCTTCCGGTTCCCCAGGACTCGGCGGAGGTTTGGTGACCGGC
CTTTTTGGAGACTGCGTAAGGCTGACGGCTGTTGTTGGAGATTTGGGCGTGGACGAAGTT
GACAATGTCAGGTCGAACTGGAGCAGTCATGACGTCTGGTAAAGTGACGGTGGTGGATTG
ATCGGTAAACGTCATTGAAAAGAATATATTGTGGTGTAAACAAATTGACGCTTAGACAAC
TTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAG
ACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCC
ACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCC
   
Sequence Name 76-K112709-SALK_029203C-R210
GenBank Accession LN486152 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K112709-SALK_029203C-R210
TTTGTTACCCACAATATATTCTTTTCATGACGTTTACCGATCAATCCACCACCGTCACTT
TACCAGACGTCATGACTGCTCCAGTTCGACCTGACATTGTCAACTTCGTCCACGCCCAAA
TCTCCAACAACAGCCGTCAGCCTTACGCAGTCTCCAAAAAGGCCGGTCACCAAACCTCCG
CCGAGTCCTGGGGAACCGGAAGAGCCGTGTCACGTATCCCTCGTGTTCCTGGTGGTGGAA
CTCACCGTGCCGGTCAAGCAGCGTTCGGAAACATGTGTCGTGGTGGTCGTATGTTTGCTC
CGACTAAGATCTGGAGACGCTGGCACCGTCGTGTCAATGTCAACATGAAGAGGCACGCGA
TTGTTTCTGCAATCGCTGCTACTGCTGTTCCAGCTCTTGTGATGGCTCGTGGTCACAAGA
TCGAGAATGTTCCTGAGATGCCTCTTGTTGTTAGCGACTCAGCTGAAGCTGTGGAGAAGA
CATCAGCTGCGATCAAGGTATTGAAGCAGATCGGTGCTTACGACGATGCGGAGAAAGCTA
AGAACAGTATTGGAATTCGTCCTGGTAAAGGTAAAATGAGGAATC

Last Updated on 06.12.2018 16:11