Sequences from confirmation amplicon


Confirmation sequences for line SALK_035172 and hit At1g26620 are shown.
Line ID SALK_035172
Locus At1g26620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:9195984
Primers used for PCR 16hd - LBb3, show primer details
   
Sequence Name 66-K112564-SALK_035172C-16hd
GenBank Accession LN485899 [GenBank]
Sequencing primer gene specific primer, 16hd: 5'-AGAAGCAATAGTTGCCATCTCAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K112564-SALK_035172C-16hd
CTTCCTCTTCACTGATGCGGCTTCAAAAGCCCGCTCTCTTGGTTTGCAGAAATAATCACT
TCTTGCGGTGATGAGTTAGAAAGAAAGATTGATGGTTCTCCAGAAGCCACAGACTTTGAA
GGTAACAGGGAAGACTATTCTTCTGGGGAGATTGATTACTTTGAAGCCATGACCCTGAAT
ATACAGGAGACAAAGGAGGAAGACTACATGCCAGAGCCATTAGTCCCAGAGAATCTGAAA
TTCGAAGATACATGTATTAACAAGCCAAGAAGAGGACAAGCAAGACGAGGAAGACCAAAG
CGGGATTTCCAAAGGGATACTCTACCTGGTCTCTCTTCTCTGTCGAGGCATGAAGTCACT
GAAGATATCCAAATGTTTGGGGGACTGATGAAAACCGGAGACTACACTTGGAGTTCTGGA
TTGGCTGTTCGACGGAACTCAAAAAGAAAACGAAATGTCACTAACATAAACCAAGCTCCA
TTATGCCCGTTGTATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTG
CGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTG
ATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCC
CAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGA
   
Sequence Name 50-K112563-SALK_035172C-R210
GenBank Accession LN485510 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K112563-SALK_035172C-R210
CATTTGTTACCCACAATACACGGGCATAATGGAGCTTGGTTTATGTTAGTGACATTTCGT
TTTCTTTTTGAGTTCCGTCGAACAGCCAATCCAGAACTCCAAGTGTAGTCTCCGGTTTTC
ATCAGTCCCCCAAACATTTGGATATCTTCAGTGACTTCATGCCTCGACAGAGAAGAGAGA
CCAGGTAGAGTATCCCTTTGGAAATCCCGCTTTGGTCTTCCTCGTCTTGCTTGTCCTCTT
CTTGGCTTGTTAATACATGTATCTTCGAATTTCAGATTCTCTGGGACTAATGGCTCTGGC
ATGTAGTCTTCCTCCTTTGTCTCCTGTATATTCAGGGTCATGGCTTCAAAGTAATCAATC
TCCCCAGAAGAATAGTCTTCCCTGTTACCTTCAAAGTCTGTGGCTTCTGGAGAACCATCA
ATCTTTCTTTCTAACTCATCACCGCAAGAAGTGATTATTTCTGCAAACCAAGAGAGCGGG
CTTTTTGAAGCCGCATCAGTTGAAGAGGAAGCTGCGTCATCAGGATGGCGTTGGTGACCA
GCCATTGAGATGGCAACTATTGCTTC

Last Updated on 06.12.2018 16:11