Sequences from confirmation amplicon


Confirmation sequences for line SALK_037612c and hit At5g04330 are shown.
Line ID SALK_037612c
Locus At5g04330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1212705
Primers used for PCR 29ke - LBb3, show primer details
   
Sequence Name 56-K113814-SALK_037612C-R210
GenBank Accession LN485646 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K113814-SALK_037612C-R210
CATTTGTTACCCACGATGGGGCAGAGGAGGCGCGTGGTCGGCACCGCCACCAAAGGAATT
GCCTTAGGAACGGTGAGACCCGGTCCTTCAACGGTGTCGACGTCACCGGGATTCATACCG
TCCGGTAAACTCCACGTGAAGCAGTGTAGAAGATGAGCCACCGCAAGCTCAAATGCGTAC
AACCCGAGTTGCATACCCGGGCACGATCTCCGACCCGACCCGAATGGAACAAATTCGAAA
TTATTCCCCTTCAGATCCGGAGCAATCGGGTTTAAAAACCTACCCGGGTTAAAACTTTCC
GGGTCGGACCAAGAATTCGGGTCACGCCCTAGAGCGTAGGTATTGACCATCACACGCGAA
CCCTTGGGGATGAAGTAACCGGAAATCTCAGTGTCCTTCACCGTCTCGTGGAGGAGGAGA
GGGAAAGGCGGGTGGAGCCGGAGGGTCTCCTTGAGGATACACTTGAGGAAATGAAGCTTC
T
   
Sequence Name 69-K113815-SALK_037612C-29ke
GenBank Accession LN485980 [GenBank]
Sequencing primer gene specific primer, 29ke: 5'-GAGAAGCTCACTTTCCTCAAGTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K113815-SALK_037612C-29ke
CGCCTTTCCTCTCCTCCTCCCGAGACGGTGAAGGACACTGAGATTTCCGGTTACTTCATC
CCCAAGGGTTCGCGTGTGATGGTCAATACCTACGCTCTAGGGCGTGACCCGAATTCTTGG
TCCGACCCGGAAAGTTTTAACCCGGGTAGGTTTTTAAACCCGATTGCTCCGGATCTGAAG
GGGAATAATTTCGAATTTGTTCCATTCGGGTCGGGTCGGAGATCGTGCCCGGGTATGCAA
CTCGGGTTGTACGCATTTGAGCTTGCGGTGGCTCATCTTCTACACTGCTTCACGTGGAGT
TTACCGGACGGTATGAATCCCGGTGACGTCGACACCGTTGAAGGACCGGGTCTCACCGTT
CCTAAGGCAATTCCTTTGGTGGCGGTGCCGACCACGCGCCTCCTCTGCCCCATCGTGGTG
TAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGG
GTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCC
TGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTG
ATGGTGGTTCCGAATCGGGCAAAA

Last Updated on 06.12.2018 16:11