Sequences from confirmation amplicon


Confirmation sequences for line SALK_043475c and hit At3g19290 are shown.
Line ID SALK_043475c
Locus At3g19290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6689107
Primers used for PCR 53aq - R204, show primer details
   
Sequence Name 65-K107371-SALK_043475C-53aq
GenBank Accession LN485864 [GenBank]
Sequencing primer gene specific primer, 53aq: 5'-AGCTATCTCAGGTTTGAGCTTCAG-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K107371-SALK_043475C-53aq
ACACATACCTATCGAGCTACAACTTAAGTTTAGTCTTTTGCTGCTGATGTTGGTCCTAAT
CAAGTATGGTCAAGGGTTTCAGAATGAACTCAATGAAAAGAAAAGTTCTTAAACAATTAC
CTCATTCTTCTGCATTTCCACCATTTCAGCCTGCAATACAGGGGAAGCTAGGATCAGAGC
CAAGTTCTAAAAGTCTGATAGGGGAAAACAGAAAACACACAGAGAGGTTGTAAACAGAAA
GAAACCTGTTTTTTCTGCAGTTCTTGATTCGTTTTCTTGAGCTTTTCAATTTCGGCTTCC
AGTTCCAATGTATAAGCCTGTAATTAGTGGATAGTTAAAAAACATGGAAGATAAAGCATC
TTTCATAATACAAGAAGTGGTTCGGGATAGAAGATATCTTTGCTGGTTTTATGTTTCTGT
TTAATGCAGTCTTGCAAAATATAAGACCATAATAGTCAAACCAAACAAAAGGTTTATATT
GTGGAAAGCCGTTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGG
ACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATT
GCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAG
   
Sequence Name 81-K107372-SALK_043475C-R210
GenBank Accession LN486229 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>81-K107372-SALK_043475C-R210
GCGTCATTTGTTACCCCAACGGCTTTCCACATATAAACCTTTTGTTTGGTTTCACTATTA
TGGTCTTATATTTTGCAAGACTGCATTAAACAGAAACATAAAACCAGCAAAGATATCTTC
TATCCCGAACCACTTCTTGTATTATGAAAGATGCTTTATCTTCCATGTTTTTTAACTATC
CACTAATTACAGGCTTATACATTGGAACTGGAAGCCGAAATTGAAAAGCTCAAGAAAACG
AATCAAGAACTGCAGAAAAAACAGGTTTCTTTCTGTTTACAACCTCTCTGTGTGTTTTCT
GTTTTCCCCTATCAGACTTTTAGAACTTGGCTCTGATCCTAGCTTCCCCTGTATTGCAGG
CTGAAATGGTGGAAATGCAGAAGAATGAGGTAATTGTTTAAGAACTTTTCTTTTCATTGA
GTTCATTCTGAAACCCTTGACCATACTTGATTAGGACCAACATCAGCAGCAAAAGACTAA
ACTTAAGTTGTAGCTCTGATAGTGTATGTTGGTCCTTATCAAGTATGTCCCCAACTGAAG
CTCAAACTGAANATAGC

Last Updated on 06.12.2018 16:11