Sequences from confirmation amplicon


Confirmation sequences for line SALK_045247 and hit At5g06670 are shown.
Line ID SALK_045247
Locus At5g06670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:2051857
Primers used for PCR 54kk - LBb3, show primer details
   
Sequence Name 70-K113914-SALK_045247C-54kk
GenBank Accession LN486007 [GenBank]
Sequencing primer gene specific primer, 54kk: 5'-ATTTATTTGATTTTGCTTTCCCCT-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K113914-SALK_045247C-54kk
AGAACTATAGAGAGTAGTCCCCTTGGGTGACAATAATGAAGGTGGAGCAGTACACCTCTC
GCAGCTGGTATAACATCTTTGTTCTTATCCTGTAATTTTATGTTCGGTTATAGCTTACTT
TTTCTTCAACTCTGGAGCATAATCAACTATATAAACTGTTTATTGTTGCACTCTTTGAAC
ACTTAAAGGTGAACAACTGATTAACTGCTCATAATTTCACTTATATCTGCAGAACCTCAT
TGACCTGGCAGGTTCTGAGAGCTCAAAAGCTGAAACCAGCGGTTTAAGGCGCAAGGAAGG
GTCTTAATAACTGTATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATG
TACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGC
CTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATC
CTGTTTGATGGTGGTTCC
   
Sequence Name 59-K113913-SALK_045247C-R210
GenBank Accession LN485718 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K113913-SALK_045247C-R210
TGAGCAGTTAATCAGTTGTTTCACCTTTAAGTGTTCAAAGAGCGCAACAATAAACAGTTT
ATATAGTTGATTATGCTCCAGAGTTGAAGAAAAAGTAAGCTATAACCGAACATAAAATTA
CAGGATAAGAACAAAGATGTTATACCAGCTGCGAGAGGTGTACTGCTCCACCTTCATTAT
TGTCACCCAAGGGACTACTCTCTATAGTCTGCAACCAAACCAGTTATAACATCTTTCAAA
AACTTTACTGATAGATATGAAAAGACAGCCAAACTGTATACTTACATTACTAAACAGGGG
AAA

Last Updated on 06.12.2018 16:11