Sequences from confirmation amplicon


Confirmation sequences for line SALK_046367c and hit At5g53840 are shown.
Line ID SALK_046367c
Locus At5g53840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:21859871
Primers used for PCR 40wj - LBb3, show primer details
   
Sequence Name 22-K118627-SALK_046367C-40wj
GenBank Accession LN484930 [GenBank]
Sequencing primer gene specific primer, 40wj: 5'-CTAGAGCATCTTGGTACTGCATGG-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K118627-SALK_046367C-40wj
TCCTTATCATATAATCAATCTTTAGAGTCAATTTCTCCAGAATTGTTGAATTCTCCAAGA
AGTACCTAGCTACTTTCAGTTCTGTTCCACATCCCAAGAGCTGACTTTCCAATTTAACAA
ACTTGAGCGACGATACCAAACACGGTGGTGGTACTGTCGAAGACATCACCTCTTCTTTTT
TATTCTTTTCATAGTCAACCAATTTCTAAGAAAACGAAAAAAAATACAAGAAAACAAGTT
AGTAATAGAGAAGCTTCACTAAAAATTGCTTTACTATTAGAGAGGGTCAAGCAATTCATC
ACCAAGCTGAGAGATTCTAGTTTCGGGCAGCTCTTAAGAAGATTTAGCAGCATTTCCAGA
TTAGAAATAGAACAAACAGCACTCAATTCGGACAGGTAACAAAATTGTTGCAAAGGTTCT
AATTCCATGTAATGAAAGATGTCCTGGTAAAAAGTAAAAATAAAGAATGTTAAGAGATAA
TATATATTTTATAGAAAAAAGATTTTGATACTCACATATATTTGTCACATGTATACCTTG
ATAGTACCATGTCTAATGACTAGAGACCTGACCCTTGAAATAGTGGTGAAGAAATCACGC
GTCATGCTTCTATTGGATAAGTCGTTTGGTGACAAATACATTGCGGACAAATTGACGCTT
AGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCAC
CAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAA
GCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCG
   
Sequence Name 11-K118626-SALK_046367C-R210
GenBank Accession LN484597 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K118626-SALK_046367C-R210
TTTGTCCGCATGTATTTGTCACCAAACGACTTATCCAATAGAAGCATGACGCGTGATTTC
TTCACCACTATTTCAAGGGTCAGGTCTCTAGTCATTAGACATGGTACTATCAAGGTATAC
ATGTGACAAATATATGTGAGTATCAAAATCTTTTTTCTATAAAATATATATTATCTCTTA
ACATTCTTTATTTTTACTTTTTACCAGGACATCTTTCATTACATGGAATTAGAACCTTTG
CAACAATTTTGTTACCTGTCCGAATTGAGTGCTGTTTGTTCTATTTCTAATCTGGAAATG
CTGCTAAATCTTCTTAAGAGCTGCCCGAAACTAGAATCTCTCAGCTTGGTGATGAATTGC
TTGACCCTCTCTAATAGTAAAGCAATTTTTAGTGAAGCTTCTCTATTACTAACTTGTTTT
CTTGTATTTTTTTTCGTTTTCTTAGAAATTGGTTGACTATGAAAAGAATAAAAAAGAAGA
GGTGATGTCTTCGACAGTACCACCACCGTGTTTGGTATCGTCGCTCAAGTTTGTTAAATT
GGAAAGTCAGCTCTTGGGATGTGGAACAGAACTGAAAGTAGCTAGGTACTTCTTGGAGAA
TTCAACAATTCTGGAGAAATTGACTCTAAAGATTGATTATATGTATAAGGATGAGGCAAA
TGTAAACCACATTCGCCAGACACTCCATGCAGTACCAAATTCCTCTA

Last Updated on 06.12.2018 16:11