Sequences from confirmation amplicon


Confirmation sequences for line SALK_053373c and hit At1g16270 are shown.
Line ID SALK_053373c
Locus At1g16270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5564423
Primers used for PCR 30ux - LBb3, show primer details
   
Sequence Name 23-K117668-SALK_053373C-R210
GenBank Accession LN484958 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K117668-SALK_053373C-R210
ACGTGGAAGTATTTTCCCACCAAAGCTACAAAGGACTTTAACCTTTGCTGTTAAACTACC
TGAGGCTGAAGAAGATGCATATCCATGGAAATTTCCTAAACTTCTATCTTGACATAATGA
AGCTTCTGGTGCTGAATGGATATGCCCGAGTTTATTTCCAAACTCGTGAACCGGGGGGTT
TGTTCTCTCATACTGCCTGATGCCATTTCCCACCATGCTCATCCTGGAAACATCTGAACC
AAATTCAGACCCTGCGTGCCCGTTATAACCTGTGGGATAATTGATATCTCCAGCTGCAAT
GGGATTTGCAGACCTTTGAGAAATGACACGATCACGCAGAAACTCAAGAGAAAATTCCTC
TCCTGTTTGTATGGAGTAATGAAGCCCCGGCTTAACATCTGCAGGATTAGGT
   
Sequence Name 60-K117669-SALK_053373C-30ux
GenBank Accession LN485750 [GenBank]
Sequencing primer gene specific primer, 30ux: 5'-GACCTAATCCTGCAGATGTTAAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K117669-SALK_053373C-30ux
AATTTTCTCTTGAGTTTCTGCGTGATCGTGTCATTTCTCAAAGGTCTGCAAATCCCATTG
CAGCTGGAGATATCAATTATCCCACAGGTTATAACGGGCACGCAGGGTCTGAATTTGGTT
CAGATGTTTCCAGGATGAGCATGGTGGGAAATGGCATCAGGCAGTATGAGAGAACAAACC
CCCCGGTTCACGAGTTTGGAAATAAACTCGGGCATATCCATTCAGCACCAGAAGCTTCAT
TATGTCAAGATAGAAGTTTAGGAAATTTCCATGGATATGCATCTTCTTCAGCCTCAGGTA
GTTTAACAGCAAAGGTTAAAGTCCTTTGTAGCTTTGGTGGGAAAATACTTCCACGTCCAG
TGAGACAACTTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGG
GGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCC
CTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTT
GATGGTGGTTCCGAAATCGGGCAAAAT

Last Updated on 06.12.2018 16:11