Sequences from confirmation amplicon


Confirmation sequences for line SALK_085866c and hit At1g03880 are shown.
Line ID SALK_085866c
Locus At1g03880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:987445
Primers used for PCR AW07 - R204, show primer details
   
Sequence Name 71-K106742-SALK_085866C-R210
GenBank Accession LN486022 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K106742-SALK_085866C-R210
CGTCATTTGTTTACCCACAATGTGTTATTAAAATTGCAAGATATATATTTAAACCAATTG
AAAACGATTTAGATTATTTAGAGTTTAAAACTCCAAATCGCAAAATCTATTAGTTTATCT
TGCCAAATCAAATTATTGCATATGTCACATAAAATTAATTTTCTAAAGATTTGTTGAGCT
TACTTTACGGATGGAGCCACGAAGAGCGCTAAGGCGGAGAAGTCTGAGGATAGGAAGATT
GTAGCTGTTAAGTGTGCTAATGTATCCGAGTGATGGCTTGTACACGTCAGCATCCGACGG
GTCATCGAGGTTTTCAGTGCATCGCATGGTGCACAAAGTCTCCTCTAAACCATTAGCTAT
TTCATGTGGTTGTTGGCCGCCTTCGCCGCGTCTCAAGGGTGGCCTAATGACGCCGAAAGG
TCCGTTGACCTTGACGATGTTGCCACGGTTATCTTGCTGGTTCTGGAGCTGCTGAGCCGT
CTCGACATTGATCTTGAAGGCTTGAGCCAAGATCTCAGGTGCGAAGCCATTGAAGATGTT
GTTTTGCTTCTGTTGCTTTCGGCCTTGTAGCCATTCCTGCCCTTGTGGGTTGTTTCCGGC
TATCAAAAATGGCTGTACACATGAAAATATATTTCT
   
Sequence Name 87-K106743-SALK_085866C-AW07
GenBank Accession LN486303 [GenBank]
Sequencing primer gene specific primer, AW07: 5'-AGTTTGGTGAATTAGTTTTGCGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>87-K106743-SALK_085866C-AW07
CCCACAAGGGCAGGAATGGCTACAAGGCCGAAAGCAACAGAAGCAAAACAACATCTTCAA
TGGCTTCGCACCTGAGATCTTGGCTCAAGCCTTCAAGATCAATGTCGAGACGGCTCAGCA
GCTCCAGAACCAGCAAGATAACCGTGGCAACATCGTCAAGGTCAACGGACCTTTCGGCGT
CATTAGGCCACCCTTGAGACGCGGCGAAGGCGGCCAACAACCACATGAAATAGCTAATGG
TTTAGAGGAGACTTTGTGCACCATGCGATGCACTGAAAACCTCGATGACCCGTCGGATGC
TGACGTGTACAAGCCATCACTCGGATACATTAGCACACTTAACAGCTACAATCTTCCTAT
CCTCAGACTTCTCCGCCTTAGCGCTCTTCGTGGCTCCATCCGTAAAGTAAGCTCAACAAA
TCTTTAGAAAATTAATTTTATGTGACATATGCAATAATTTGATTTGGCAAGATAAACTAA
TAGATTTTGCGATTTGGAGTTTTAAACTCTAAATAATCTCAATCGTTTTCCATTGGTTTA
AATATATATCCTGCAATTTTAATAACACATTGTGGTGTCAATAAATTGT
Primers used for PCR 54xv - LBb3, show primer details
   
Sequence Name 39-K119638-SALK_085866C-54xv
GenBank Accession LN485291 [GenBank]
Sequencing primer gene specific primer, 54xv: 5'-ACTATATTTAGCCCAAAGTCTCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K119638-SALK_085866C-54xv
TTTTTTTCTTTTTATTTAATAGTAACGTTTATATAATCCTAAAAGATGTAGAAATTAAAG
ACTTAAAGATTTTTTATTCAGGAAAGAGTTACATGTGCAGAGTCAACGATCGCTTATACA
CGACGTTCGTTGTTGTACACTAGACGTCCTAATACAAAGCTCAAAACAAAACAGTTCCCT
TAAGCGTAGAACTTCTTTGTACATTATTATTTCATATTACAAGTCGTACCAAACCGAACT
TTAGATTCAAACGCTATTCCACCACGCGCTCATCAAGCCTCAAGCCCTAGGCCTTCCGTA
GCTCATTGGACTGCTATGGGTCAGTGTGGTCTCAATCGTGCTAAACTTTACTCGTTTAGC
TTCTTCGGGAGAGATCTGGTACCCATTGGTTATAACCTCAAGCGGCAAACCTCTCATGAC
TGAGGTACGGCCCGCGAGTGTGTTGACCTGTGCGTTTTCGTTTGTCTTGAATTCGATCCA
CTCGAACTGTTCGCCTATGGCATGTTTCATGACCGAAAAGCCTTGTGGCACGACTAGTAA
CTGTCCGCTGGAGATCTCTTGGTCGAACACTCTTTCTCCGTTGTCGTTCACCATTTGTAT
ATGAGCCTTTCCGTTTGTCACGTAGAGTGCCGCGTTTGCGTTTACGTTCCATTGCGGTAG
CACCATAGCGTTCTGCGTGCAAGATATATTTTTTAAATTGAAAACGATTAAAAATGCAAG
ATATATATATATATATATATTTTTTAATTAACGCTTTATTTTTTATATTGTGGTGTAAAC
AAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAGTGTACTGGGGTGGT
TTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTT
   
Sequence Name 37-K119637-SALK_085866C-R210
GenBank Accession LN485260 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K119637-SALK_085866C-R210
GTCATTTGTTACACCACAATATAAAAAATAAAGCGTTAATTAAAAAATATATATATATAT
ATATATCTTGCATTTTTAATCGTTTTCAATTTAAAAAATATATCTTGCACGCAGAACGCT
ATGGTGCTACCGCAATGGAACGTAAACGCAAACGCGGCACTCTACGTGACAAACGGAAAG
GCTCATATACAAATGGTGAACGACAACGGAGAAAGAGTGTTCGACCAAGAGATCTCCAGC
GGACAGTTACTAGTCGTGCCACAAGGCTTTTCGGTCATGAAACATGCCATAGGCGAACAG
TTCGAGTGGATCGAATTCAAGACAAACGAAAACGCACAGGTCAACACACTCGCGGGCCGT
ACCTCAGTCATGAGAGGTTTGCCGCTTGAGGTTATAACCAATGGGTACCAGATCTCTCCC
GAAGAAGCTAAACGAGTAAAGTTTAGCACGATTGAGACCACACTGACCCATAGCAGTCCA
ATGAGCTACGGAAGGCCTAGGGCTTGAGGCTTGATGAGCGCGTGGTGGAATAGCGTTTGA
ATCTAAAGTTCGGTTTGGTACGACTTGTAATATGAAATAATAATGTACAAAGAAGTTCTA
CGCTTAAGGGAACTGTTTTGTTTTGAGCTTTGTATTAGGACGTCTAGTGTACAACAACGA
ACGTCGTGTATAAGCGATCGTTGACTCTGCACATGTAACTCTCTCCTGAATAAAAAATCT
TTAAGTCTTTAATTTCTACATCTTTTAGGATTATATAAACGTTACTATATAAATAAAAAA
GAAAAAAAAATCAGTTCACTAACATGCGAGACTTGG

Last Updated on 06.12.2018 16:11