Sequences from confirmation amplicon


Confirmation sequences for line SALK_087836c and hit At5g40710 are shown.
Line ID SALK_087836c
Locus At5g40710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:16295541
Primers used for PCR CF10 - R204, show primer details
   
Sequence Name 44-K105819-SALK_087836C-CF10
GenBank Accession LN485365 [GenBank]
Sequencing primer gene specific primer, CF10: 5'-AAGTTTTGGAAAAGGGTATAGTCCA-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K105819-SALK_087836C-CF10
TCAATTGAGAGAAATAGTTTAAAGCATTTAAAGGTTTGAATGAATTCTAACCAAATGTGT
ACAAAAAAAATTCATTATTGTATCTTCATATTTTTTAGTGAAATTAAATAACATTGGATT
TTTTCCTTGAATACAAAAAACATCCAAATCCATTAAAGACTAATATTCAATAACACCACC
TATAAATTCTTATGTTTTGAAAATAACCCCAAACTATTTTTCTTTCTTAACCCTTAAAGT
TTCATTTTTCATATATTACACCAAAATTTGAAATCATCTTCTTTGATTGTTTTTGGTGTG
AGCGAAGATGGGAAGATCGAATTCTCAATGCCCATCTCATTGGATTCTCCTCTGCTTCTT
CATCTCTTCTCAGTTTTGGGGATTCTCACTTCCCACTTCGATAATTCAACAAAATCTCGA
GGTGAAATTTTTAGGGTTTTTCAGCAATTTCAACTGTAAATTTCGTGTAGAAGAATGTCT
GTATCACTTTAGGGTTTGTAATCGATTTGGCTTACTTGGGATTTACTTAGAATTCTAGAT
TTTGTTCAAATTTTGAAGCTTTGTGGTTGTTGTTCTACAGGGTTTTAAAGATCCTGAAGA
CGGTTTTCATGAGATTCATTGTTCAAGAGAACGTAGCAGAGTTGCTTGGAAAATCATTCA
AGAGGTATGAATTGAAAGCAGTGGTTTTTATTATTTTTTATGCAAATCTGTAGAGTTTTC
TCATTGATCTTTGTTGTTGCTGTGAAGTATCTCATGCCAATTGACGCANATTGTGGTGTA
AACAAATTGACGCTNAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGT
GGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCC
   
Sequence Name 60-K105820-SALK_087836C-R210
GenBank Accession LN485729 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K105820-SALK_087836C-R210
GTCATTTGTTTACCACAATTTGCGTCAATTGGCATGAGATACTTCACAGCAACAACAAAG
ATCAATGAGAAAACTCTACAGATTTGCATAAAAAATAATAAAAACCACTGCTTTCAATTC
ATACCTCTTGAATGATTTTCCAAGCAACTCTGCTACGTTCTCTTGAACAATGAATCTCAT
GAAAACCGTCTTCAGGATCTTTAAAACCCTGTAGAACAACAACCACAAAGCTTCAAAATT
TGAACAAAATCTAGAATTCTAAGTAAATCCCAAGTAAGCCAAATCGATTACAAACCCTAA
AGTGATACAGACATTCTTCTACACGAAATTTACAGTTGAAATTGCTGAAAAACCCTAAAA
ATTTCACCTCGAGATTTTGTTGAATTATCGAAGTGGGAAGTGAGAATCCCCAAAACTGAG
AAGAGATGAAGAAGCAGAGGAGAATCCAATGAGATGGGCATTGAGAATTCGATCTTCCCA
TCTTCGCTCACACCAAAAACAATCAAAGAAGATGATTTCAAATTTTGGTGTAATATATGA
AAAATGAAACTTTAAGGGTTAAGAAAGAAAAATAGTTTGGGGTTATTTTCAAAACATAAG
AATTTATAGGTGGTGTTATTGAATATTAGTCTTTAATGGATTTGGATGTTTTTTGTATTC
AAGGAAAAAATCCAATGTTATTTAATTTCACTAAAAAATATGAAGATACAATAATGAATT
TTTTTTGTACACATTTGGTTAGAATTCATTCAAACCTTTAAATGCTTTAAACTATTTTCT
CTTCAATTGATTTTTTAATCTTTTTTTGAATAATGGACTATAC
Primers used for PCR 81xo - LBb3, show primer details
   
Sequence Name 55-K119292-SALK_087836C-81xo
GenBank Accession LN485633 [GenBank]
Sequencing primer gene specific primer, 81xo: 5'-TAAATGCGATTGTACCGCTCTAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K119292-SALK_087836C-81xo
TCTAGTACATATTGGTGTAGTAGTGATATAATTGTAAGGGATGTTGCAAAATGCACTAAT
CTGATCCTAATTGTAATCATCTTGCAAAGAAAAAAAATGTACTCTCTCTCAGCCGCCATT
AAAGCTAAAGCTTGATATGGTTAGAAGGGTTTCTTCTTCAGACCATTGTGTCTAATACGC
TTCAAATCTTGAGATCTTCTCTTTAATCCTCTACAGATTAGTAAAACGTAAGAACAATGA
GTTCATGAGGGCCTTTTTCGGTTTTAGAGTTGTAAGGAAATCTGAAACAGTATTTTAATG
TTGTTGTTACCTTCGGAACAAATAGACAAAGCTGTAATAGAGAAGAAGCACTACCAAAAC
GATGATGGAGAAAATAATGTACACTATACTCCTCTTCTGCAAAACATATGACTGAGATTA
AATGAGATATGATATGATATAATATCATTTACAAAAAAAGGGTTTATTATGTTACCTTTG
GTTTTTGAGATAAGGGCTTTGATCCTCCTGAACATGTATGTGCATCGCAGAACTGACGCA
AGAAGAAATCTGCAGTTTTAAAAGTTAAAACCCCCAAAAAAGGGTGGTGAATCTCTATGG
ATCATTAAAACCCCCCACGAAGAAGTCTACAATTGTAAAGTTTAAACCCCAAAAGTAGTG
GTGAATCTTTATGGATCAATACAAGCAAAAACTGTTGAAATAAGATAGTAAAATTCAGGA
AGGTTCTAATACCGTGAAGACGGTTGGCTGATGAACCTTTATTTACCGGAAAACAACTAT
TAGCCAAACTCTGCAATGCAACCACACCAGAGCAGATAAGCACGGGAACACAAGAAACAG
AACGAAGACAATATGTTCAGAAGTGTTCAAATCTTTCA
Primers used for PCR CF11 - LBb3, show primer details
   
Sequence Name 54-K119290-SALK_087836C-CF11
GenBank Accession LN485612 [GenBank]
Sequencing primer gene specific primer, CF11: 5'-GTTATGAATCGCTAATAGACCCCA-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K119290-SALK_087836C-CF11
GATGGATCATTTTACAATAATGCATCAGTATATACCGCATTAAGTAAATTGTAATGGCGC
GAATCGAAATGCTTATCGAGATACTTCTCTTCATAAAAAGCCTTTTTGCAGAATCCACAA
CGCCACTCATTAATATCTGAACGCAATTTGTGCTCTTCTTGTTCTCTATAAATGTCATTG
TCTCGATGAACTCTACAAGTACTCGGTAAACAACAACAAAAAAACAAATTGCATAACACA
TTGACATGCTGGAAACAAATTGACGCTTAGACAACTTGACGCTTAGACAACTTAATAACA
CATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAAC
AGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTT
TGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAAACCGGCAAAA
   
Sequence Name 06-K119289-SALK_087836C-R210
GenBank Accession LN484446 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K119289-SALK_087836C-R210
GCGTCAATTTGTTTCCGCATGTCAATGTGTTATGCAATTTGTTTTTTTGTTGTTGTTTAC
CGAGTACTTGTAGAGTTCATCGAGACAATGACATTTATAGAGAACAAGAAGAGCACAAAT
TGCGTTCAGATATTAATGAGTGGCGTTGTGGATTCTGCAAAAAGGCTTTTTATGAAGAGA
AGTATCTCGATAAGCATTTCGATTCGCGCCATTACAATTTACTTAATGCGGTATATACTG
ATGCATTATTGTTAAAATGATCCATCTTTCTGTGACAATTCATACTGTTTTGGGGTCTAT
TAGCGATTCATAAC

Last Updated on 06.12.2018 16:11