Sequences from confirmation amplicon


Confirmation sequences for line SALK_089894c and hit At5g16070 are shown.
Line ID SALK_089894c
Locus At5g16070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:5248311
Primers used for PCR 02ii - LBb3, show primer details
   
Sequence Name 36-K112623-SALK_089894C-02ii
GenBank Accession LN485235 [GenBank]
Sequencing primer gene specific primer, 02ii: 5'-GTGGAGCAAGTGAAGAATCCTAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K112623-SALK_089894C-02ii
ACTTTATGTCTTCATTAAGTTTTGATGGTTCATTTGTTCTTTTAGTAGTTTTAGTGTTCA
TTCATGAATTTCTTGGGTTTTGTAGGGCCTAATGATCACACTATTGCCCAAATTAAGGAC
GCTGTTCGTGATGGTCTGAGATCGGTCAAGAACACCATAGAAGACGAGTGTGTTGTGTTA
GTAAGTACCTTATATATCTTGAAGTTTTTTTGTTAACATTGCTTTATGTTTCTCCGCAAT
AACACATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGT
GGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTG
AGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGAT
GGTGGTTCCGAAATCGGCAAAA
   
Sequence Name 12-K112622-SALK_089894C-R210
GenBank Accession LN484614 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K112622-SALK_089894C-R210
TGCGGAGAACATAAAGCAATGTTAACAAAAAAACTTCAAGATATATAAGGTACTTACTAA
CACAACACACTCGTCTTCTATGGTGTTCTTGACCGATCTCAGACCATCACGAACAGCGTC
CTTAATTTGGGCAATAGTGTGATCATTAGGCCCTACAAAACCCAAGAAATTCATGAATGA
ACACTAAAACTACTAAAAGAACAAATGAACCATCAAAACTTAATGAAGACATAAAAGTCG
TCCTAATACCTTTTATGAGGATGGTACATGAATTAGGATTCTTCACTTGCTCCAC

Last Updated on 06.12.2018 16:11