Sequences from confirmation amplicon


Confirmation sequences for line SALK_092343c and hit At4g31360 are shown.
Line ID SALK_092343c
Locus At4g31360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:15222302
Primers used for PCR CE47 - R204, show primer details
   
Sequence Name 49-K107002-SALK_092343C-R210
GenBank Accession LN485470 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K107002-SALK_092343C-R210
GCGTCATTTGTTACGCAAAAATTACCAGTGTTCTATAACAATCTTGGTTCTCGTAGGGTC
CTCAACCTCTGGCTCTTCCTTCTCAACCTCCTCCTCAACGGCTTCCTCTGGCTCTTCAAC
TTCCTCTTCCTTCTTTCCCTTCTTGGCTCTCCCAGTCTCAATCGCTGAATTCTTCCTCTT
AGCCTTCTTCGTCGCCGGCTTAGTGGCGGATCCAGCCGCTTTAGCACCGTTTTGTTGAGT
CTGACTTCGAGTTCGACTGTCCATGCTCCGAGTCACTCTAGACGACGCCAACGTCGTTAA
AGGTTTCGCCTTTTCTTCTCCATCTGCCTTACTCTTCTTCGGCGGCATTGTACGGACAGC
AACAGGGTTTCGTCTTAACAATCTTCTTCTCCTTCTTCTCCGTCTCTGTCTTACCGCTCT
TTCTTACTTTTTTGTTGTTTGATATTCCCTTTTGTGTTTGCTTTTTTCTTACCTTGGAAA
AGTAATTCGAATAAACGG
   
Sequence Name 57-K107003-SALK_092343C-CE47
GenBank Accession LN485655 [GenBank]
Sequencing primer gene specific primer, CE47: 5'-CCGTTTATTCGAATTACTTTTCCA-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K107003-SALK_092343C-CE47
ACCACAGGGATATCGACACAAAAAAGTAAGAAAGAGCGGTAAGACAGAGACGGAGAAGAA
GGAGAAGAAGATTGTTAAGACGAAACCCTGTTGCTGTCCGTACAATGCCGCCGAAGAAGA
GTAAGGCAGATGGAGAAGAAAAGGCGAAACCTTTAACGACGTTGGCGTCGTCTAGAGTGA
CTCGGAGCATGGACAGTCGAACTCGAAGTCAGACTCAACAAAACGGTGCTAAAGCGGCTG
GATCCGCCACTAAGCCGGCGACGAAGAAGGCTAAGAGGAAGAATTCAGCGATTGAGACTG
GGAGAGCCAAGAAGGGAAAGAAGGAAGAGGAAGTTGAAGAGCCAGAGGAAGCCGTTGAGG
AGGAGGTTGAGAAGGAAGAGCCAGAGGTTGAGGACCCTACGAGAACCAAGATTGTTATAG
AACACTGGTAATTTTTGCGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGG
ACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATT
GCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACG
Primers used for PCR CE48 - LBb3, show primer details
   
Sequence Name 70-K119298-SALK_092343C-CE48
GenBank Accession LN486019 [GenBank]
Sequencing primer gene specific primer, CE48: 5'-TGAAAGCATTGCATTGTTTACTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K119298-SALK_092343C-CE48
AAACAAAAGAACTCTTGTGAGACATAAGTATAGAAGCAAATTCCATTTATAGAATGAACA
CGTCCATTACGCATTGCAATTCAGCTAAAGCAAGACAAGAACACAAACAGAAAGCAGATA
GTCAAGTCCTCTTCACAAATGCGAGCAAAACATAAACCACTAATCACTCAAACCAAAAAC
TTATGCAGAATCAAGATGAAAACCTAACACATTGTTCCACCCTAGCAAATAAACCTTAGC
TGCTACACACGGACACACAAGTCTCAACACATACCCCAAAGTTGTAGGCTTTTAGATTCA
ATTTCAAAGAAAAAAACCTAAATTTTTGCTCTTATGATTCCAAACATTATCTTGTGTAAA
CAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGG
TTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAG
AGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGG
TGGTTCCGAAATCGGCAAAA
   
Sequence Name 22-K119297-SALK_092343C-R210
GenBank Accession LN484934 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K119297-SALK_092343C-R210
CATTTGTTTACCAGATAATGTTTGGAATCTAAGAGCAAAAATTTAGGTTTTTTTCTTTGA
AATTGAATCTAAAAGCCTACAACTTTGGGGTATGTGTTGAGACTTGTGTGTCCGTGTGTA
GCAGCTAAGGTTTATTTGCTAGGGTGGAACAATGTGTTAGGTTTTCATCTTGATTCTGCA
TAAGTTTTTGGTTTGAGTGATTAGTGGTTTATGTTTTGCTCGCATTTGTGAAGAGGACTT
GACTATCTGCTTTCTGTTTGTGTTCTTGTCTTGCTTTAGCTGAATTGCAATGCGTAATGG
ACGTGTTCATTCTATAAATGGAATTTGCTTCTATACTTATGTCTCACAAGAGTTCTTTTT
TGTTTTGTTTTGTGATGTTATTGAAGTAAACAATGCAATGCTTTC

Last Updated on 06.12.2018 16:11