Sequences from confirmation amplicon


Confirmation sequences for line SALK_108664 and hit T10K17 are shown.
Line ID SALK_108664
Locus T10K17 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21425166
Primers used for PCR 24rb - LBb3, show primer details
   
Sequence Name 82-K116055-SALK_108664C-24rb
GenBank Accession LN486246 [GenBank]
Sequencing primer gene specific primer, 24rb: 5'-GTGATCAAAGCCTACCTGAGTTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K116055-SALK_108664C-24rb
GCACAGATCTTACCGTTACATATTGCATAATAAGAACATGAGTTCAGATAGTAATTTTTT
TTGTATATAACTATTATCAGTTTATCACCATATATAATATGTTTATTAGTCCCAAGCATA
ACAAAGATCATATTCTCGAGTCTGTGCATCAAGAGCACAAGCTCCATACACTATGATGGA
CCAAAATCAGTTTCAGCACTGATATTTGTCAATATTATCTCTGCTATCTTTATAAATAGT
AATACGTTGTGCTTTATGACCAAATATAAACATACAAACGTTACGAGTTAGTCCCCGTAA
ACTTTTCCTAAACTGAACGAATTCCGTCGTACGCCCCGGTAAAAATACTTGGTCTTAATA
ATTCCCATTACTACGACAACTAGAATTTAGCACCAAACCATCTCCGATATCGTTTGTGAT
GTATACATATATTTTCAAAAGAAAAATCCGATTTGAATATATTCTCACCCATCACTACAC
CGTTTTGAGATAATGCTACTTTCATAAGCAAGGATAGTATCACAAGCATTATCAGTTTTC
TATGGGAAGCCATCTTTTCTCTTCTTTATGTTATGGTTTGCAATGTTTCGTTGAGGTTCG
TTTTCTATTCATATATTTATAGGCTTGTTAATGAATTTGTTTAGACCATGTTAAGTTGTA
ACGTACCGTGCGTTAACTTAAAACATTGACTTGCTAGCTTGGGGGTTTATGATCTAATAA
CCGTTTTGCATTCTATTGAAACACACATTATTGTTAATGCTACCATTAAAAAATCTAATT
GTAGGTTAAAGGCTTAAAGCGACTTGATTTGTTATTCTAGAAAGAGGGTCAAGGCTTATA
TGTTAAACCCAAGAAACAATAAAGTACTACAAATTGACGGAATATATNGTGTCAACAAAT
TGCGGTAAACAAATTGCGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTAC
TGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTG
GCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCT
   
Sequence Name 55-K116054-SALK_108664C-R210
GenBank Accession LN485628 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K116054-SALK_108664C-R210
TGTTGACCAATATATCCCGTCAATTTGTATTACTTTATTGTTTCTTGGGTATAACATATA
AGCCTTGATCCCTCTTTCTAGAATAACAAATCAAGTCGCTTTAAGCCTTTAACCTACAAT
TAGATTTTTTAATGGTAGCATTAACAATAATGTGTGTTTCAATAGAATGCAAAACGGTTA
TTAGATCATAAACCCCCAAGCTAGCAAGTCTATGTTTTAAGTTAACGCACGGTACGTTAC
AACTTAACATGGTCTAAACTAATTCATTAACAAGCCTATGAATATATGAATAGAAAACGA
ACCTCAACGAAACATTGCAAACCATAACATAAAGAAGAGAAAAGATGGCTTCCCATAGAA
AACTGATAATGCTTGTGATACTATCCTTGCTTATGAAAGTATCATTATCTCAAAACGGTG
TAGTGATGGGTGAGAATATATTCAAATCGGATTTTTCTTTTGAAAATATATATATACATC
ACAAACGATATCGGAGATGGTTTGGTGCTAAATTCTAGTTGTCGTAGTAATGGGAATTAT
TAAGACCAATTATTTTTACCGGGGCGTACTACGGAATTCGTTCAGTTTAGGAAAAGTTTA
CGGGGACTAACTCGTAACGTTTGTATGTTTATATTTGGTCATAAAGCACAACGTATTACT
ATTTATAAAGATAGCAGAGATAATATTGACAAATATCAGTGCTGAAACTGATTTTGGTCC
ATCATAGTGTATGGAGCTTGTGCTCTTGATGCACAGACTCAAGAATATGATCTTTGTTAT
GCTTGGGACTAATAAACATATTATATATGGTGATAAACTGATAATAGTTATATACAAAAA
AAATTACTATCTGAACTCTTGTTCTTATTA

Last Updated on 06.12.2018 16:11