Sequences from confirmation amplicon


Confirmation sequences for line SALK_112206 and hit At4g02880 are shown.
Line ID SALK_112206
Locus At4g02880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:1276024
Primers used for PCR 10dv - LBb3, show primer details
   
Sequence Name 59-K109940-SALK_112206C-10dv
GenBank Accession LN485710 [GenBank]
Sequencing primer gene specific primer, 10dv: 5'-CTCCTCCACCTTGTCAAGAGTATC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K109940-SALK_112206C-10dv
CTTTCTGCCTCCTTCTCTTGAAGTTAACTTCTCTCATCAGATTCATAATCGACTCCATCA
CAGTGAACAAGGTTCTCTACATCATCACAAAATATAAAGAAGAAAATGATACTGAGATAT
CGATAAAGAAACCCTCCTATCGAACACATAACCCCAAAATTAATTATTTCCTGGAAATGA
AAGAATGTCCAAAATATACACTGGCATACCTTGTTACTTTTGGCATCTTCAATGATCTGT
TCAAGGTGGACCATGTTGCATCCCTGGGTTGATCTGTTACCAACAGAGCTAACAGCACCA
TCCAGCTCTGGATCACCATTTTCATCGGCCAAAGAGCCCTTTGATATCTCATCCACAAGA
TTTGAACCACTCAACTCGGCATCACTATTTTCAACAGCCAACGAACCACTTGATGTCTCG
TTCACAACATCGATACTCCCAAATCTAATTTGA
   
Sequence Name 50-K109939-SALK_112206C-R210
GenBank Accession LN485503 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K109939-SALK_112206C-R210
GTCATTTGTTATATGATTGGCAAGGTGTTGAGTTTCACAGTACAGGTAATCAAGCAGAAG
CTAGCACAAGCGCAGATTCAGAAGATGCTGTGCATAAGCTGGTTTATCCTGCAGACAATT
TGGCAATTACACAGAACTCACATCCTCTTCAAATTAGATTTGGGAGTATCGATGTTGTGA
ACGAGACATCAAGTGGTTCGTTGGCTGTTGAAAATAGTGATGCCGAGTTGAGTGGTTCAA
ATCTTGTGGATGAGATATCAAAGGGCTCTTTGGCCGATGAAAATGGTGATCCAGAGCTGG
ATGGTGCTGTTAGCTCTGTTGGTAACAGATCAACCCAGGGATGCAACATGGTCCACCTTG
AACAGATCATTGAAGATGCCAAAAGTAACAAGGTATGCCAGTGTATATTTTGGACATTCT
TTCATTTCCAGGAAATAATTAATTTTGGGGTTATGTGTTCCAAAGGAGGGTTTCTTTATC
GATATCTCAATATCAATTTCCTCCTTATATTTTGG

Last Updated on 06.12.2018 16:11