Sequences from confirmation amplicon


Confirmation sequences for line SALK_121111c and hit At4g40000 are shown.
Line ID SALK_121111c
Locus At4g40000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:18544875
Primers used for PCR 45dr - LBb3, show primer details
   
Sequence Name 77-K110311-SALK_121111C-45dr
GenBank Accession LN486170 [GenBank]
Sequencing primer gene specific primer, 45dr: 5'-GAGCATGTTTCCTTCTGGTAAAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>77-K110311-SALK_121111C-45dr
TGAAGAATGGCTTCTATCTCTTCTGATGAATCAGCCGAAGAAGTCTGTGATCTTCCCCTT
GAACTTTGTATGAGGATACTGCCTCATGATCAAAACACTGGGGGATTTTTCATTGCAGTC
CTTCACAAAGTTTCACCTTTACCAGGTGAATTAGAAGAACTTTCTGTTGCATAACCTCTC
TAGTTGATTCCTTAATACGAATTCCGGATCATTTTCTTGCAGACTTTCAGGAGAAAGTAA
CTCAGAAAAGGAATTTGTCTACTAGAAACAACATCAACTCATCTGAGAAGTCTTCTTCGT
ATGAAGCAGTATCTGACACTGTGGTTACTAAACCTGAAGAGGGTACAGAGGAAATAGTTT
TAGAAGCAACAGTTTCAGAGAATGGCTTTGAGCCGCCGGAGATGGAAAGCACCTGGAATG
AAGGTATCGTAGAATTGGCTCAAAAACTTGACGCTTAGACAACTTAATAACACATTGCGG
ACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATT
GCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAG
CAGGCGAAAATCCTGTTTGATGGTGGTTCCGA
   
Sequence Name 67-K110310-SALK_121111C-R210
GenBank Accession LN485923 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K110310-SALK_121111C-R210
GCGTCAGTTTTTGAGCCATTCTACGATACCTTCATTCCAGGTGCTTTCCATCTCCGGCGG
CTCAAAGCCATTCTCTGAAACTGTTGCTTCTAAAACTATTTCCTCTGTACCCTCTTCAGG
TTTAGTAACCACAGTGTCAGATACTGCTTCATACGAAGAAGACTTCTCAGATGAGTTGAT
GTTGTTTCTAGTAGACAAATTCCTTTTCTGAGTTACTTTCTCCTGAAAGTCTGCAAGAAA
ATGATCCGGAATTCGTATTAAGGAATCAACTAGAGAGGTTATGCAACAGAAAGTTCTTCT
AATTCACCTGGTAAAGGTGAAACTTTGTGAAGGACTGCAATGAAAAATCCCCCAGTGTTT
TGATCATGAGGCAGTATCCTCATACAAAGTTCAAGGGGAAGATCACAGACTTCTTCGGCT
GATTCATCAGAAGAGATAGAAGCCATTTCTTCATAGCTGTTTTTGCCACCCGATGAGTCT
TTATCACTTTTACCAGAAGGAAACATGCT

Last Updated on 06.12.2018 16:11