Sequences from confirmation amplicon


Confirmation sequences for line SALK_133861c and hit At1g62950 are shown.
Line ID SALK_133861c
Locus At1g62950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:23315366
Primers used for PCR 64cs - LBb3, show primer details
   
Sequence Name 09-K109680-SALK_133861C-64cs
GenBank Accession LN484516 [GenBank]
Sequencing primer gene specific primer, 64cs: 5'-CTGAAATACATTGATGGAAGGGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K109680-SALK_133861C-64cs
ATAATGCTTGATGGTACAGACAAACAGACACCTTCTTCTCCCACTCTTAAATTACACACA
CTTACTTACACACACATAAGCTTGCACGTGGTTCTTGTTCACATGGTGGAAGCCACAGAT
GTATATAGAGAGACAGAGAGAGAGAGGAAACAAAACCAAACCTTTTGCTGCAGAAGAGAC
AGACTATTAAATTGCTTCACCTGCTTTACTTACTTTCCAGCCAAGGCACACTCTCCACGC
ACTCATCTTTTCTCAAATCTCTCTCTATATAGATTTGTGTCCAGAAATTAATGGGTTTGG
TTCGGAAACTTATGACTGGTAATACCCAGATCTTATTCTATGTTTCTTGAAACTCTCTTC
TCGAGGCAAATGCAGCTACTTCTTCATGCTTTTGTTCTTCTTCTTCTTCGTTTATCAAGA
AATTGTTAACGAAATAGCTTAACCGGGTTTGAGTTCGGTTTAGGATCCGATAACCGCAAA
TGCAGATTGGGAAATTGATTTGGGTAATAATGTTCATATTCGTTCACATTATTATTACAT
CATCTCGAAGTTTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTT
TAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTC
ACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGA
AAATCCTGTTTGATGGTGGTTCCG
   
Sequence Name 25-K109679-SALK_133861C-R210
GenBank Accession LN484998 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K109679-SALK_133861C-R210
GTCATTTGTTTAACTTCGAGATGATGTAATAATAATGTGAACGAATATGAACATTATTAC
CCAAATCAATTTCCCAATCTGCATTTGCGGTTATCGGATCCTAAACCGAACTCAAACCCG
GTTAAGCTATTTCGTTAACAATTTCTTGATAAACGAAGAAGAAGAAGAACAAAAGCATGA
AGAAGTAGCTGCATTTGCCTCGAGAAGAGAGTTTCAAGAAACATAGAATAAGATCTGGGT
ATTACCAGTCATAAGTTTCCGAACCAAACCCATTAATTTCTGGACACAAATCTATATAGA
GAGAGATTTGAGAAAAGATGAGTGCGTGGAGAGTGTGCCTTGGCTGGAAAGTAAGTAAAG
CAGGTGAAGCAATTTAATAGTCTGTCTCTTCTGCAGCAAAAGGTTTGGTTTTGTTTCCTC
TCTCTCTCTGTCTCTCTATATACATCTGTGGCTTCCACCATGTGAACAAGAACCACGTGC
AAGCTTATGTGTGTGTAAGTAAGTGTGTGTAATTTAAGAGTGGGAGAAGAAGGTGTCTGT
TTGTCTTGTACCATCAAGCATTTATTCTTTGTTGCCTACACCAATCAAACCCTTCCATCA
ATGTATTTCAG

Last Updated on 06.12.2018 16:11