Sequences from confirmation amplicon


Confirmation sequences for line SALK_134490c and hit At1g03340 are shown.
Line ID SALK_134490c
Locus At1g03340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:819965
Primers used for PCR 51wu - LBb3, show primer details
   
Sequence Name 45-K118859-SALK_134490C-51wu
GenBank Accession LN485405 [GenBank]
Sequencing primer gene specific primer, 51wu: 5'-TTTCTCCATCTTGTTGAGTTCCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K118859-SALK_134490C-51wu
AATCATTCATTCAGCTCTGCTACTTGAGAACAGAATGTTAGCATCCACCATGAGATCCAA
TAACGGCTTTGCATATGGTTGCTGAGAACTTGATCTTAAAGCATCTAGACCCATCAATTC
AGATAGGAGAGACAAATCGAATCCTTCTTCTTTGAGTTCTTCTTCTGATCTAGACAAAAG
GAACTCTAACACCTCCTCTTTTCTTGCATACTGCTGAGTGATCCCCAAGCTTAAACACAC
AGGGTCTGGTCCAGTTTCTGTTAACCACAGTTCATGTCAGTCGAAAAAAGGGATTACAAT
TTGGTGAATTCAACTGAAATTTGATCAAAGTCACATACCATGAACATCCATGAGTCTATG
CTTCATCATGGTTGAATCAAACTCCAAAAAGTGGTTAGACTTACTAAAAGAAGAAAAAGG
TTTCAATGGCTCCAAGAGTTGTGATTTCAGGATATATTGTGGTGTAAACAAATTGACGCT
TAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCA
CCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCA
AGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTC
   
Sequence Name 21-K118858-SALK_134490C-R210
GenBank Accession LN484900 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K118858-SALK_134490C-R210
GTCATTTGTTTACCCACAATATATCCTGAAATCACAACTCTTGGAGCCATTGAAACCTTT
TTCTTCTTTTAGTAAGTCTAACCACTTTTTGGAGTTTGATTCAACCATGATGAAGCATAG
ACTCATGGATGTTCATGGTATGTGACTTTGATCAAATTTCAGTTGAATTCACCAAATTGT
AATCCCTTTTTTCGACTGACATGAACTGTGGTTAACAGAAACTGGACCAGACCCTGTGTG
TTTAAGCTTGGGGATCACTCAGCAGTATGCAAGAAAAGAGGAGGTGTTAGAGTTCCTTTT
GTCTAGATCAGAAGAAGAACTCAAAGAAGAAGGATTCGATTTGTCTCTCCTATCTGAATT
GATGGGTCTAGATGCTTTAAGATCAAGTTCTCAGCAACCATATGCAAAGCCGTTATTGGA
TCTCATGGTGGATGCTAACATTCTGTTCTCAAGTAGCAGAGCTGAATTGAATGATTTGGT
CTCTACGGCAGCTGAGTTTCATAGATTAAGGAACTCAACAAAATGGAGAAA

Last Updated on 06.12.2018 16:11