Sequences from confirmation amplicon


Confirmation sequences for line SALK_134899 and hit At4g08430 are shown.
Line ID SALK_134899
Locus At4g08430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:5349928
Primers used for PCR CG75 - R204, show primer details
   
Sequence Name 56-K107002-SALK_134899C-R210
GenBank Accession LN485635 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K107002-SALK_134899C-R210
TGTGTTTTCTACCCACAGCTTCTCAATTCCAGGCACACTCTCATATATCCACACCAGCAA
GGCCTGAACACAACCGTGAATCACATAAGAATCCCCGTCATACTTGACAAACTTCACAGA
CCTTAATAGAGAGTCAAAAGCCACTCGACCCCAAGGATACTTCTCAAAAGCAACAGGATC
AAGCACTCTTTTAGCACTCGACAACGGGACTCTAGAACCGTGATGAATCCCATGTACTCC
CACATATTGTAAACATAATCTACCAACCATCATCCTCTTCTCCAAACTCCATGTCTTGCT
AATCTCAAAGACACGTTCCAACTCTGTAAACAATGGACCAACAGACATTGCTACCCCCAT
TTCATTCCAAAAATCTTTGTAATCAGTTTCACCAGTGTCAGACTCGTCAAACATATCGCA
ATTGAGGCCCGTTATGTTCTCAAACTCGTAGAGAGAAAATCTTATTGGCCTGCGATCAAT
CAAACACCACATCTCATGCATGTTGTCTACTCGCAACTGATTCGTGAGGAAGAAATGAAC
TGTCTGGGTAG
   
Sequence Name 64-K107003-SALK_134899C-CG75
GenBank Accession LN485836 [GenBank]
Sequencing primer gene specific primer, CG75: 5'-GCTACCCAGACAGTTCATTTCTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K107003-SALK_134899C-CG75
CATGCATGAGATGTGGTGTTTGATTGATCGCAGGCCAATAAGATTTTCTCTCTACGAGTT
TGAGAACATAACGGGCCTCAATTGCGATATGTTTGACGAGTCTGACACTGGTGAAACTGA
TTACAAAGATTTTTGGAATGAAATGGGGGTAGCAATGTCTGTTGGTCCATTGTTTACAGA
GTTGGAACGTGTCTTTGAGATTAGCAAGACATGGAGTTTGGAGAAGAGGATGATGGTTGG
TAGATTATGTTTACAATATGTGGGAGTACATGGGATTCATCACGGTTCTAGAGTCCCGTT
GTCGAGTGCTAAAAGAGTGCTTGATCCTGTTGCTTTTGAGAAGTATCCTTGGGGTCGAGT
GGCTTTTGACTCTCTATTAAGGTCTGTGAAGTTTGTCAAGTATGACGGGGATTCTTATGT
GATTCACGGTTGTGTTCAGGCCTTGCTGGTGTGGATATATGAGAGTGTGCCTGGAATTGG
AGAAGCTTGTGGGTTTAGAAAAACAACAAATTAATAACACATTGCGGACGTTTTTAATGT
ACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCC
TGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACG
Primers used for PCR CG76 - LBb3, show primer details
   
Sequence Name 80-K118526-SALK_134899C-CG76
GenBank Accession LN486226 [GenBank]
Sequencing primer gene specific primer, CG76: 5'-TTATGAGATACATCATCTGCAGGC-3'
Sequence (A. th genome BLAST matches underlined) 
>80-K118526-SALK_134899C-CG76
ATCCTCGTTGATGATTCTTGATTCTGTTTCCTCTTATGTCGTGCTGCCACATGTGTCCAC
GCATGAGGTTATAAACGGTTGTGGATGATGTCTTTAAGCAAGTTGTCAAGCGCCGTATCA
TGATTCTCAGCTGAGTCACTCCATTTAGGATACATGTTTTCTTCAGATACAAGGATCATA
TGGCTCACACGCACCTACATACATATAATCACAAGATTAATGTAAAACGTTTGTGCAAGT
TCAGTTTCAGTTTTTTTTTAAAGACAAGTAGCAGATTTGATCTAGAAAACCTTACATACC
TGTCCGTGGGCAGCCTTCTCCTTTTCAATGAAAGCGGTAAAGTTAAAACGTTTTTTAGAA
GATCTCCAATCAAGTAGCGGAACACCCGTCACAACTTGACCGAATATGTCCTGCAGGATA
TATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTA
ATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCAC
CGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAA
ATCCTGTTTGATGGTGGTTCCGAATCGGGCAAA
   
Sequence Name 70-K118525-SALK_134899C-R210
GenBank Accession LN486017 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K118525-SALK_134899C-R210
CATTTGTTTACCCACAATATATCCTGCAGGACATATTCGGTCAAGTTGTGACGGGTGTTC
CGCTACTTGATTGGAGATCTTCTAAAAAACGTTTTAACTTTACCGCTTTCATTGAAAAGG
AGAAGGCTGCCCACGGACAGGTATGTAAGGTTTTCTAGATCAAATCTGCTACTTGTCTTT
AAAAAAAAACTGAAACTGAACTTGCACAAACGTTTTACATTAATCTTGTGATTATATGTA
TGTAGGTGCGTGTGAGCCATATGATCCTTGTATCTGAAGAAAACATGTATCCTAAATGGA
GTGACTCAGCTGAGAATCATGATACGGCGCTTGACAACTTGCTTAAAGACATCATCCACA
ACCGTTTATAACCTCATGCGTGGACACATGTGGCAGCACGACATAAGAGGAAACAGAATC
AAGAATCATCAAACGAGGATGGTGAGAGTAAAGTGATGGAATCAACGCCTGCAGATGATT
ATTCTCATA

Last Updated on 06.12.2018 16:11