Sequences from confirmation amplicon


Confirmation sequences for line SALK_145681 and hit At5g47110 are shown.
Line ID SALK_145681
Locus At5g47110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:19134627
Primers used for PCR CG19 - LBb3, show primer details
   
Sequence Name 65-K107792-SALK_145681C-CG19
GenBank Accession LN485866 [GenBank]
Sequencing primer gene specific primer, CG19: 5'-TATATCCATGGCGTTATTCTCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K107792-SALK_145681C-CG19
TCTCATCCCCAGATCTCAACCTCTCTTCTCTCCACCAAGCGTTTCTCTCTAATCTCCGTC
CCTAGAGCTTCCTCCGACAATGGTACGACTTCCCCCGTCGTGGAGATTCCGAAGCCTGCT
TCTGTGGCTGTAGAGGAAGTTCCAGTTAAATCTCCAGCCGAAAGCTCCTCCGCTTCTGAA
AACGGCGCCGTTGGAGGTGAAGCGACTGATTCGAGTACTGAGACGGTAATCAAATATCAG
AATGCGAAGTGGGTTAATGGAACTTGGGATCTGAAACAGTTCGAGAAAGATGGCAAAACT
GATTGGGATTCTGTTATCGTTTCTGGTTAGATCCTTTTAAAACTTCAGTTCAGTACAGAT
TCTTTGAATACTTGATATATGTCTATGCTTTGTTATTAGGAAGATTCTGCTTTGGTTTCT
TATAGTGGTGTGTAAGAAGAGGAGTGTTTGTTGTGTAAATGTGTTGATTATGTATTTTTA
TAGAGGCAAAGAGGAGAAAATGGCTTGAAGATAACCCGGAAACAACGAGTAACGACGAGC
TGGATGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGT
ACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCC
TGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCC
TGTTTGATGGTGGTTCCGA
   
Sequence Name 16-K107793-SALK_145681C-R210
GenBank Accession LN484713 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K107793-SALK_145681C-R210
GCGTCATTTGTTACATCCAGCTCGTCGTTACTCGTTGTTTCCGGGTTATCTTCAAGCCAT
TTTCTCCTCTTTGCCTCTATAAAAATACATAATCAACACATTTACACAACAAACACTCCT
CTTCTTACACACCACTATAAGAAACCAAAGCAGAATCTTCCTAATAACAAAGCATAGACA
TATATCAAGTATTCAAAGAATCTGTACTGAACTGAAGTTTTAAAAGGATCTAACCAGAAA
CGATAACAGAATCCCAATCAGTTTTGCCATCTTTCTCGAACTGTTTCAGATCCCAAGTTC
CATTAACCCACTTCGCATTCTGATATTTGATTACCGTCTCAGTACTCGAATCAGTCGCTT
CACCTCCAACGGCGCCGTTTTCAGAAGCGGAGGAGCTTTCGGCTGGAGATTTAACTGGAA
CTTCCTCTACAGCCACAGAAGCAGGCTTCGGAATCTCCACGACGGGGGAAGTCGTACCAT
TGTCGGAGGAAGCTCTAGGGACGGAGATTAGAGAGAAACGCTTGGTGGAGAGAAGAGAGG
TTGAGATCTTGGGGATGAGATTAGGGTTCTGAAGTGAGGAAGAGATCGGCGGAGAGAATA
ACGCCATGGATATA
Primers used for PCR 62wt - R212, show primer details
   
Sequence Name 28-K118709-SALK_145681C-62wt
GenBank Accession LN485096 [GenBank]
Sequencing primer gene specific primer, 62wt: 5'-CTAAGGTCCCTACTAATTTTGGGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K118709-SALK_145681C-62wt
AATTGAGTCAAATTAGTTGCGGCAATAAGAGCAGAGCAGTAATGTGTTACACCCTTAAAG
GACGTTAGTACTGAAGCCCAAATTCTTTGGAGGTTGGTTGGTTGGTTGATGAAGTGCGGT
TTTGATTAATTCACAAATATGACTTACTTGATGACTTTTCACAAACATCCATCTGGAAAC
GCTACTATTTGATGATGTATTAACAGTAGAAAGCATATTAAAAAAAATAAAATCTCTTAT
TGATTAAATTATCAAGCGTCAAAACGAAATAGGTCTGCTTTACAAGTCAAAGACCTTCGA
TACATTATCTTTGAGACTACTTCATAGAGATTTTTAAAGCAGAAAGATTACAAAATCTGA
AGATCAGTAGTTCAGTAGAGATAAACTTTCACTGATTCACAGATTATTGCATAACAAAAA
AAGGAAAATGAAAGATTGTAAGAACTTGTTCACTTCTTTGAAGAAACTGTTGATGAATCT
GGCTCTTTCCATGCAGCTTGCCATTGTTTGTCATATAACGTAGTCTCATCGAACAGATCC
TTAAGTTTGTCTAAATCTTCATTCTTACGGATGAAGAGAACTCCAGCTACAGCCACAAAC
AAGAGTGTTTTGCAGAAGAAATTTCCCATTTGATCAACAAGTCCTACTCCGGTAAGACTA
TCAACAAAGTAAGCCATGAAGAACCCTATCATCGCAGCACGACCTACACCACCAAGACAA
CAACTTAGATGCACAAAATGTTAAGTGTACTATATCTAAGTGTTAGATCATAAGTGTTTT
GAAAATCACCATTGAGAAGTTCAGCTTCAGGTAGATGGTATCTCTTCATCCATGCCCA
   
Sequence Name 03-K118708-SALK_145681C-R214
GenBank Accession LN484354 [GenBank]
Sequencing primer gene specific primer, R214: 5'-AACGTTGCGGTTCTGTCAGTTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K118708-SALK_145681C-R214
GCGGGGGTCATAACGTGACTCCCTTAATTCTCCGCTCATGATCAGATTGTCGTTTCCCGC
CTTCAGTTTAAACTATCGATTGTCGGTACCACCATGGATACTTCGATTATTCCATGGTGG
GCATGGATGAAGAGATACCATCTACCTGAAGCTGAACTTCTCAATGGTGATTTTCAAAAC
ACTTATGATCTAACACTTAGATATAGTACACTTAACATTTTGTGCATCTAAGTTGTTGTC
TTGGTGGTGTAGGTCGTGCTGCGATGATAGGGTTCTTCATGGCTTACTTTGTTGATAGTC
TTACCGGAGTAGGACTTGTTGATCAAATGGGAAATTTCTTCTGCAAAACACTCTTGTTTG
TGGCTGTAGCTGGAGTTCTCTTCATCCGTAAGAATGAAGATTTAGACAAACTTAAGGATC
TGTTCGATGAGACTACGTTATATGACAAACAATGGCAAGCTGCATGGAAAGAGCCAGATT
CATCAACAGTTTCTTCAAAGAAGTGAACAAGTTCTTACAATCTTTCATTTTCCTTTTTTT
GTTATGCAATAATCTGTGAATCAGTGAAAGTTTATCTCTACTGAACTACTGATCTTCAGA
TTTTGTAATCTTTCTGCTTTAAAAATCTCTATGAAGTAGTCTCAAAGATAATGTATCGAA
GGTCTTTGACTTGTAAAGCAGACCTATTTCGTTTTGACGCTTGATAATTTAATCAATAAG
AGATTTTATTTTTTTTAATATGCTTTCTACTGTTAATACATCATCAAATAGTAGCGTTTC
CAGATGGATGTTTGTGAAAAGTCATCAAGTAAGTCATATTTGTGAATTATCAAAACCGCA
CTTCATCAACCAACCAACCAACCTCCAAAGAATTTGGGCTTCAGTA

Last Updated on 06.12.2018 16:11