Sequences from confirmation amplicon


Confirmation sequences for line SALK_150241c and hit At2g30290 are shown.
Line ID SALK_150241c
Locus At2g30290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:12912923
Primers used for PCR 29eb - LBb3, show primer details
   
Sequence Name 23-K119050-SALK_150241C-29eb
GenBank Accession LN484963 [GenBank]
Sequencing primer gene specific primer, 29eb: 5'-ATGTGAATGAGTGTGAGGAGAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K119050-SALK_150241C-29eb
GGAGCTATGAATGTAGTTGCAGCGGAAGCTTGCTTTACATAAGAGAACACGACATTTGCA
TAAGTATATTACACTCATCATTCATCATATTTTCGAATAAACGAATCATTCACTAATCAT
TAACTTGTGTACAGATAGAGATGCAAGAGGAGATTTCAGTTGGGGAGTGATATGGATAAT
AATAATGGGATTAGGTGCAGCTGCTTTAGGAGCTTACACTGTTTATAAATACAGAATTCG
GGTAATCAGATATCTCAAATCATTATAATACTTTGTTCATTAATATTGGATATACACATA
TTTGATTGATTTGTGTGTGTATGTGTGTGCAGACATATATGGACTCAGAGATAAGAGCTA
TAATGGCACAATACATGCCTAGACAACTTAGACAACTTAATAACACATTGCGGACGTTTT
TAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTC
ACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGA
AAATCCTGTTTGATGGTGGTTCCGAAATCGGCAAAAT
   
Sequence Name 07-K119049-SALK_150241C-R210
GenBank Accession LN484478 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K119049-SALK_150241C-R210
ATTGTGCCATTATAGCTCTTATCTCTGAGTCCATATATGTCTGCACACACATACACACAC
AAATCAATCAAATATGTGTATATCCAATATTAATGAACAAAGTATTATAATGATTTGAGA
TATCTGATTACCCGAATTCTGTATTTATAAACAGTGTAAGCTCCTAAAGCAGCTGCACCT
AATCCCATTATTATTATCCATATCACTCCCCAACTGAAATCTCCTCTTGCATCTCTATCT
GTACACAAGTTAATGATTAGTGAATGATTCGTTTATTCGAAAATATGATGAATGATGAGT
GTAATATACTTATGCAAATGTCGTGTTCTCTTATGTAAAGCAAGCTTCCGCTGCAACTAC
ATTCATAGCTTCCCCATGTGTTTTTGCATTTGCAATCGCGACATTGGCACGCTGTTTTCT
CCTCACACTCATTCACAT
Primers used for PCR CF81 - LBb3, show primer details
   
Sequence Name 83-K121396-SALK_150241C-CF81
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, CF81: 5'-GTGTAGAGAAAAGCGATTTTGGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>83-K121396-SALK_150241C-CF81
ATTTTTCAAACGTCGGCGTTTTAAAGGTGGAGTAAATTAAGCCCAAACCGTCGAAGCCTG
TTTATTTTTTCCACAAGCAAAATATGGCTTCAGAAATGTTTGATTTGGCTAAAATCAGAG
TTTTATAAAGTTTATAACTAAAAAGCAGAAACAAAATTCATGATAACAAAAATAAATTAA
AATCTGATTCAACATATTTTGCATTTTCTCTTTGCATTGTTAATCCATTCTTAGGTAGGC
AAGTTTTCACTATTTGGTTGTGTGTACATGATGTTTATTAATATATATGAACACAAAATA
AAATAACAAATATATAAAACCTGCCCACACTATCAAATGGACTTGACTAATATGGTTTTA
TAGTGTTGAATCTGTGATATTAAAATGACAAAAACTTATAATCAAGCGTAGAACTCTATA
TATGAGATCAAAGTTTGAACTTTGAAGTTTGATGTTGAATTTGGTGAACACATTGTTCTT
ACAACTCTAGTTGAGAAGAAAGTTGAGTATTGGTGTAAACAAATTGACGCTTAGACAACT
TAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGA
CGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCA
CGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCC
   
Sequence Name 45-K121395w-SALK_150241C-R210
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K121395w-SALK_150241C-R210
CAATACTCAACTTTTCTTTCTTAACTAGAGTAGTAAGAACAAGGTGTTTCACCAAATTTC
AACATCAAACTTCAAAGTTCAAACTTTGATCTCATATATAGAGTTCTACGCTTGATTATA
AGTTTTTGTCATTTTAATATCACAGATTCAACACTATAAAACCATATTAGTCAAGTCCAT
TTGATAGTGTGGGCAGGTTTTATATATTTGTTATTTTATTTTGTGTTCATATATATTAAT
AAACATCATGTACACACAACCAAATAGTGAAAACTTGCCTACCTAAGAATGGATTAACAA
TGCAAAGAGAAAATGCAAAATATGTTGAATCAGATTTTAATTTATTTTTGTTATCATGAA
TTTTGTTTCTGCTTTTTAGTTATAAACTTTATAAAACTCTGATTTTAGCCAAATCAAACA
TTTCTGAAGCCATATTTTGCTTGTGGAAAAAATAAACAGGCTTCGACGGTTTGGGCTTAA
TTTACTCCACCTTTTAAAACGCCGACGTTTTGAAAAATAAGAAAACGCGGCGTTTTTGTT
TCCTCCCCGTCTCCAAAATCGC

Last Updated on 06.12.2018 16:11