SimpleSearch - Line and FST details
Line specific information
| Line ID | 220E03 |
| Vector Used | pAC161 |
| Line Availability | available as T3 set from NASC (N421075) |
| Segregation Analysis | 50:7:7 |
| Confirmed for Hit | At1g27780 |
| Parent of DUPLO pair | none |
| Parent of pair(s) | none |
| Note |
Gene hit At4g05280
| Sequence (A. th genome BLAST matches underlined) | >21-K014203-022-220-E03-8409 NTTGGATCCATGTAGATTTGCCTGACATGAATGCCTTTACAATTGACACTCATACCCTAT GGGATCCTCCCTATAGTGAGCATCACAAATAAGTATGTTAACCATAGATAATGTACATTT ACTTATTATGTACGTACATACACAATTTAACACACCTCATCAGCAGCCTCACACTCCAAA ATGCGAGATGTCCGGATTGCTCGCAGAGAGGCAAGACGATGGATAGACCTCTCAGCAAAT GTCCTGTTAGGTACATCATCAGGTCCTATGGGATCCTCCCTATAGTGAG |
| GenBank Accession | AL767590 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr4:2698940 - go to primer design |
| BLAST e Value | 3e-100 |
| Hit Clone Code (BAC ID) | C17L7 |
| Hit Gene Code | At4g05280 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | |
| Insertion Classification | TS2TE |
| Confirmation Status | failed |
Gene hit At1g20580
| Sequence (A. th genome BLAST matches underlined) | >21-K014205-022-220-E03-8409 GTCTATCTTTTTCTTTTACTGGTGATACTCCCTATGGAGAGACGAGCTTACCTGTTTTGA AGCTATGGTGACTGATTGCATGGGCTTTATATATGCCCATATTCATATTAATGCCCAATG CGAGAAACTAGCTTTTTAGTCCCTATACTTGGACATTATTTTAATGCTTATTCTACGACA GACGACGGGAGACCTCACGGGTTAAAACGAAGGAACCACCGGTACCAAAACTCGCCGGAA GAGATGGCGGACGAGATGGAGACCAAAGGAGAGAAGAAGTACAAGTATAGCATAATCATT CCTACCTACAACGAGCGCCTCAACATCGCTATCATAGTCTACCTGATCTTCAAGCATCTC CGGTAAATGAATATTTATTTCTTTTGGTCTTTTCACGTTCATTCTTTCATTTCAGAATCC GAATTACGTTTCTTGTTATTCTTCTACTGAATACTCGAACCTGATCGACAATTTCAAGTT ACGGATCTCGGAAATTTCCCGATCATGTTTCT |
| GenBank Accession | AL767591 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr1:7128896 - go to primer design |
| BLAST e Value | 0.0 |
| Hit Clone Code (BAC ID) | F5M15 |
| Hit Gene Code | At1g20580 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | Small nuclear ribonucleoprotein family protein |
| Insertion Classification | TS2TE (5') |
| Confirmation Status | unknown |
Gene hit At1g27780
| Sequence (A. th genome BLAST matches underlined) | >21-K014203-022-220-E03-8409 NTTGGATCCATGTAGATTTGCCTGACATGAATGCCTTTACAATTGACACTCATACCCTAT GGGATCCTCCCTATAGTGAGCATCACAAATAAGTATGTTAACCATAGATAATGTACATTT ACTTATTATGTACGTACATACACAATTTAACACACCTCATCAGCAGCCTCACACTCCAAA ATGCGAGATGTCCGGATTGCTCGCAGAGAGGCAAGACGATGGATAGACCTCTCAGCAAAT GTCCTGTTAGGTACATCATCAGGTCCTATGGGATCCTCCCTATAGTGAG |
| GenBank Accession | AL767590 [GenBank] |
| Graphic View | 
|
| Predicted Position of Insertion | Chr1:9678402 - go to primer design |
| BLAST e Value | 3e-100 |
| Hit Clone Code (BAC ID) | T22C5 |
| Hit Gene Code | At1g27780 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | |
| Insertion Classification | TS2TE |
| Confirmation Status | confirmed, show confirmation sequences |
| Primer and wt-amplicons | show primer details |
|
Last Updated on 10.06.2021 13:37 |
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