SimpleSearch - Line and FST details
Line specific information
| Line ID | 270E04 |
| Vector Used | pAC161 |
| Line Availability | available as T3 set from NASC (N425876) |
| Segregation Analysis | 43:29:21 |
| Confirmed for Hit | At4g35190 |
| Parent of DUPLO pair | none |
| Parent of pair(s) | none |
| Note | |
Gene hit F17A8
| Sequence (A. th genome BLAST matches underlined) | >84-K020162-022-270-E04t-8409 TCATACCGAGGACCAAAGTTTTGGGGTTTGGAGAATGCTCTATGGGATCCTCCCTATAGT TAGAAGANCATCCTAACGTTCTTATCCTTATCGCTATGAGGATCCTCACTATAGTGAGCT GCTATGGTATCCTCCCTATAGTGAGGAGNTNNNNGTATCNNAGTTANTTTTACCGGAATA TGTTCCATCCAATACAATAAACTCAACCAAAATTTCTC |
| GenBank Accession | FR806931 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr4:6141944 - go to primer design |
| BLAST e Value | 5e-13 |
| Hit Clone Code (BAC ID) | F17A8 |
| Insertion Classification | Genome Hit |
| Confirmation Status | unknown |
Gene hit F26G5
| Sequence (A. th genome BLAST matches underlined) | >84-K020163-022-270-E04t-8409 AGTGTAATGATGCACATCCTGAAGCATCAAGAGAAGATACTACTATAATCACATGTCACA ATCATACATATACATTAAGATTTGTTCAGGGTTTTCTTCATACCGAGGACCAAAGTTTTA AGGTTTGGAGAATGCCCTATGGGATCCTCCCTATAGTGAG |
| GenBank Accession | BX530850 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr3:15847821 - go to primer design |
| BLAST e Value | 1e-57 |
| Hit Clone Code (BAC ID) | F26G5 |
| Insertion Classification | Genome Hit |
| Confirmation Status | failed |
Gene hit At5g65510
| Sequence (A. th genome BLAST matches underlined) | >29-K015063-022-270-E04-8409 TATCATGAAATTTCCNGGACATGAAGCATTTACAATTGATATATGGTACTGTTCTTCTTG CACATAGCGTTTAACAGATATAACTGTGAAATAGCTATATAACTGTTTAGACTTTATTTT TCTCTATGGTATCGACCCTAAGATGAGATAAATCTGGCTCACTCGCAAGTTCCAGATTTA AGTACCAAAAGACATCAAAGTTCTATGGGATCCTCCCTATAGAGAGATCTTTGAAAATCT TTCATTTCACCTTCTATGGGGACCCCAATGCTGATGTCTGTATGATGCAATCTCTGTTTC TTGATTGACATGTATTTGGACCCACTTTTGACCCTTTATTCCAATGCTGTTATATGACAA TAATGACATGCATGCATATATTAATAAATACATGTGGTGTCAGACTAAACACTATGCATA TATTCTTTTGCTATGGGATCCT |
| GenBank Accession | CT026217 [GenBank] |
| Graphic View |
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| Predicted Position of Insertion | Chr5:26187607 - go to primer design |
| BLAST e Value | 3e-106 |
| Hit Clone Code (BAC ID) | K19O4 |
| Hit Gene Code | At5g65510 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | AINTEGUMENTA-like 7 |
| Insertion Classification | CDSi |
| Confirmation Status | failed |
Gene hit At4g35190
| Sequence (A. th genome BLAST matches underlined) | >29-K015070-022-270-E04-8409 NTGATCCATGTAGAATTTCCCGGACATGAAGCACTTACAATTGAATATATCCTGAAACTT TAGGAATCGATCCTGTATTAGAGGATATAATTATGAGAAATATTAAAGTTTGTTGTTTTC TGGGGTTTCTTTTGTCCCTCTTGAAGAAACCACCTGTATCAAACTTTCAACAAACTGATT CCACCAAAATGCACAACCTGAAAATATCAGCACTGAATCTTGGCCAATTCTTTGTAGAAA ATGAACACACAATCTCTTGTGCTGTATACCAAAGAGTTCATTCCCTCAATTATGAAGATC AGAAAGAACAACACAAACCATCACAATGATATTTTGTGGTTGCAGCGGGATCCAACTTTC CCCAAATCTTACGGAGCAGGAATAGGCTGTTCCATTGGGTCGGAGAAACTCTCTGCTCTG ATGAATTTGATATATAAATATATAATTGATACTATATTTTTTGGTACCGAAAGACTTTTT AATTGAACCCGATAAAATCAACTTCAAAAACTTTAATTAAACCGAACCTGAACCGGTTTT GACTATGAAACCGAGATATATAGTGGCTTTCGGGTCCCAGTTTCTTTGGGTCCTATGGGA TCCT |
| GenBank Accession | AL942964 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr4:16748109 - go to primer design |
| BLAST e Value | 0.0 |
| Hit Clone Code (BAC ID) | T12J5 |
| Hit Gene Code | At4g35190 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | Putative lysine decarboxylase family protein |
| Insertion Classification | TS2TE (3') |
| Confirmation Status | confirmed, show confirmation sequences |
| Primer and wt-amplicons | show primer details |
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Last Updated on 10.06.2021 13:37 |
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