Genotyping and primer details


Genotyping and primer details for SALK_130243 - At2g39690
Line ID SALK_130243
Locus At2g39690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:16541611 of line SALK_130243 Chr2:16541611
   
Insertion allele amplicon LBb3 - 23uh
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 23uh: 5'-AGACTTAATACCTCAAGCGTGGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:16542166
   
wt amplicon (over InsPos) 57vr - 23uh
wt amplicon start Chr2:16540798
wt amplicon end Chr2:16542166
wt amplicon size 1369 bp
Locus specific primer 23uh: 5'-AGACTTAATACCTCAAGCGTGGAA-3'
2nd locus specific primer 57vr: 5'-CGCCAAGTCTACTCTTTTTATCAGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11