Genotyping and primer details


Genotyping and primer details for SALK_072339c - At3g58050
Line ID SALK_072339c
Locus At3g58050 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:21495894 of line SALK_072339c Chr3:21495894
   
Insertion allele amplicon LBb3 - 26ie
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 26ie: 5'-ACTCTCTCTTCCTTCAGATTGCTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:21496298
   
wt amplicon (over InsPos) 05jw - 26ie
wt amplicon start Chr3:21495268
wt amplicon end Chr3:21496298
wt amplicon size 1031 bp
Locus specific primer 26ie: 5'-ACTCTCTCTTCCTTCAGATTGCTG-3'
2nd locus specific primer 05jw: 5'-TTCAAGAAAGACAGTGTTTGGATG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11