Genotyping and primer details


Genotyping and primer details for 205G08 - At1g03060
Line ID 205G08
Locus At1g03060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:725075 of line 205G08 Chr1:725075
   
Insertion allele amplicon 8474 - MZ78
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MZ78: 5'-CACGGAAATCTCACATAGTTTCAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:724558
   
wt amplicon (over InsPos) 10sq - MZ78
wt amplicon start Chr1:724558
wt amplicon end Chr1:725477
wt amplicon size 920 bp
Locus specific primer MZ78: 5'-CACGGAAATCTCACATAGTTTCAA-3'
2nd locus specific primer 10sq: 5'-GTCCAGGATCCTTACTCTTTGATG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11