Genotyping and primer details


Genotyping and primer details for 723A03 - At1g04410
Line ID 723A03
Locus At1g04410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:1189745 of line 723A03 Chr1:1189745
   
Insertion allele amplicon 8474 - GE61
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer GE61: 5'-TCAATCCCTGAAAAGAACATCTCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:1190247
   
wt amplicon (over InsPos) 05dk - GE61
wt amplicon start Chr1:1189200
wt amplicon end Chr1:1190247
wt amplicon size 1048 bp
Locus specific primer GE61: 5'-TCAATCCCTGAAAAGAACATCTCT-3'
2nd locus specific primer 05dk: 5'-CCAATTTTCTTCTTTTCATGGGTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11