Genotyping and primer details


Genotyping and primer details for 007G05 - At1g06960
Line ID 007G05
Locus At1g06960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:2137835 of line 007G05 Chr1:2137835
   
Insertion allele amplicon 8474 - EV10
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer EV10: 5'-GAGAGAAATGAAAAGAGCTCCAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:2138460
   
wt amplicon (over InsPos) 22hf - EV10
wt amplicon start Chr1:2137226
wt amplicon end Chr1:2138460
wt amplicon size 1235 bp
Locus specific primer EV10: 5'-GAGAGAAATGAAAAGAGCTCCAGA-3'
2nd locus specific primer 22hf: 5'-ATCCTTGTTTCCTAATACCCGTCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11