Genotyping and primer details


Genotyping and primer details for 789E07 - At1g07030
Line ID 789E07
Locus At1g07030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:2159571 of line 789E07 Chr1:2159571
   
Insertion allele amplicon 8474 - 32fp
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 32fp: 5'-TATATCAAGCGATGCTGTTTTCAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:2160102
   
wt amplicon (over InsPos) 07gi - 32fp
wt amplicon start Chr1:2159027
wt amplicon end Chr1:2160102
wt amplicon size 1076 bp
Locus specific primer 32fp: 5'-TATATCAAGCGATGCTGTTTTCAC-3'
2nd locus specific primer 07gi: 5'-CAAGTAAAAACATTTAAGCGGAGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11