Genotyping and primer details


Genotyping and primer details for 144G09 - At1g09920
Line ID 144G09
Locus At1g09920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:3226535 of line 144G09 Chr1:3226535
   
Insertion allele amplicon 8474 - FK20
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer FK20: 5'-TTCTGAAATTTCGTTTCTTCAGGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:3226953
   
Insertion allele amplicon 3144 - KB54
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer KB54: 5'-GGAGCATGTGTGTTCAAGTAGTGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:3225995
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11