Genotyping and primer details


Genotyping and primer details for 050C01 - At1g10450
Line ID 050C01
Locus At1g10450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:3434625 of line 050C01 Chr1:3434625
   
Insertion allele amplicon 3144 - 07uv
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 07uv: 5'-AGGAAGGACAATAGCCACACTAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:3434846
   
wt amplicon (over InsPos) 47vl - 07uv
wt amplicon start Chr1:3434320
wt amplicon end Chr1:3434846
wt amplicon size 527 bp
Locus specific primer 07uv: 5'-AGGAAGGACAATAGCCACACTAGA-3'
2nd locus specific primer 47vl: 5'-GACCACTTGGTATCTGTCACTTCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11