Genotyping and primer details


Genotyping and primer details for 225A03 - At1g13620
Line ID 225A03
Locus At1g13620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:4668439 of line 225A03 Chr1:4668439
   
Insertion allele amplicon 8474 - 14sa
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 14sa: 5'-AGTTGGATTGGCTCAGTTTCTTTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:4668186
   
wt amplicon (over InsPos) 36sq - 14sa
wt amplicon start Chr1:4668186
wt amplicon end Chr1:4668867
wt amplicon size 682 bp
Locus specific primer 14sa: 5'-AGTTGGATTGGCTCAGTTTCTTTA-3'
2nd locus specific primer 36sq: 5'-ATTCACTACCTTTGCATTTGAACC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11