Genotyping and primer details


Genotyping and primer details for 932G05 - At1g13880
Line ID 932G05
Locus At1g13880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:4750557 of line 932G05 Chr1:4750557
   
Insertion allele amplicon 8474 - 42sf
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 42sf: 5'-TTCATTTACTTCCCTCTGGAGAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:4750972
   
wt amplicon (over InsPos) 12tc - 42sf
wt amplicon start Chr1:4750162
wt amplicon end Chr1:4750972
wt amplicon size 811 bp
Locus specific primer 42sf: 5'-TTCATTTACTTCCCTCTGGAGAAA-3'
2nd locus specific primer 12tc: 5'-ATGAAGAACAAGTGACAGGGAAGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11