Genotyping and primer details


Genotyping and primer details for 213B06 - At1g15730
Line ID 213B06
Locus At1g15730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:5407720 of line 213B06 Chr1:5407720
   
Insertion allele amplicon 8474 - 10dr
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 10dr: 5'-CACCGTTGAAGAAAGGAAGATTAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:5407102
   
wt amplicon (over InsPos) 09ej - 10dr
wt amplicon start Chr1:5407102
wt amplicon end Chr1:5408041
wt amplicon size 940 bp
Locus specific primer 10dr: 5'-CACCGTTGAAGAAAGGAAGATTAG-3'
2nd locus specific primer 09ej: 5'-ACCTCGAGAAGGTATTAACCCAAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11