Genotyping and primer details


Genotyping and primer details for 290G04 - At1g17720
Line ID 290G04
Locus At1g17720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:6094875 of line 290G04 Chr1:6094875
   
Insertion allele amplicon 8474 - CQ40
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CQ40: 5'-GCAGCTTCAGTACTTCCTTGAGAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:6094368
   
wt amplicon (over InsPos) 17gf - CQ40
wt amplicon start Chr1:6094368
wt amplicon end Chr1:6095062
wt amplicon size 695 bp
Locus specific primer CQ40: 5'-GCAGCTTCAGTACTTCCTTGAGAC-3'
2nd locus specific primer 17gf: 5'-TTGTCAGAAATGACTTGGAAATGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11