Genotyping and primer details


Genotyping and primer details for 800D11 - At1g18610
Line ID 800D11
Locus At1g18610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:6405696 of line 800D11 Chr1:6405696
   
Insertion allele amplicon 8474 - 26gz
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 26gz: 5'-AGACATGCAATATGGATGAACAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:6406087
   
wt amplicon (over InsPos) 14ix - 26gz
wt amplicon start Chr1:6405420
wt amplicon end Chr1:6406087
wt amplicon size 668 bp
Locus specific primer 26gz: 5'-AGACATGCAATATGGATGAACAAA-3'
2nd locus specific primer 14ix: 5'-TGTAACGTAAATCCTTAAGCCTCG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11