Genotyping and primer details


Genotyping and primer details for 107B08 - At1g18890
Line ID 107B08
Locus At1g18890 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:6524708 of line 107B08 Chr1:6524708
   
Insertion allele amplicon 3144 - DL95
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer DL95: 5'-CACTAAAGGCTATTGATTTTGGCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:6525129
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11