Genotyping and primer details


Genotyping and primer details for 951B07 - At1g23550
Line ID 951B07
Locus At1g23550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:8352183 of line 951B07 Chr1:8352183
   
Insertion allele amplicon 8474 - 21kz
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 21kz: 5'-GTGCTAAAGGATTGGTTGGTGTAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:8351868
   
wt amplicon (over InsPos) 11ua - 21kz
wt amplicon start Chr1:8351868
wt amplicon end Chr1:8352724
wt amplicon size 857 bp
Locus specific primer 21kz: 5'-GTGCTAAAGGATTGGTTGGTGTAT-3'
2nd locus specific primer 11ua: 5'-AAGGGATCCTCTGTTTTTACCACT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11