Genotyping and primer details


Genotyping and primer details for 448E01 - At1g23900
Line ID 448E01
Locus At1g23900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:8442985 of line 448E01 Chr1:8442985
   
Insertion allele amplicon 8474 - 35rb
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 35rb: 5'-CATACAGGAGCATCGACGTAATTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:8442698
   
wt amplicon (over InsPos) 32rt - 35rb
wt amplicon start Chr1:8442698
wt amplicon end Chr1:8443387
wt amplicon size 690 bp
Locus specific primer 35rb: 5'-CATACAGGAGCATCGACGTAATTT-3'
2nd locus specific primer 32rt: 5'-GTACGGCAAAAACTCAGTAACCAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11