Genotyping and primer details


Genotyping and primer details for 053D01 - At1g30680
Line ID 053D01
Locus At1g30680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:10882719 of line 053D01 Chr1:10882719
   
Insertion allele amplicon 8474 - PF68
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer PF68: 5'-CTTGATATGTCCGACGGTATTTCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:10882296
   
Insertion allele amplicon 3144 - PF69
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer PF69: 5'-TCGTAAGCTACCTCGTCACCTATT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:10883113
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11