Genotyping and primer details


Genotyping and primer details for 759F03 - At1g31280
Line ID 759F03
Locus At1g31280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:11182449 of line 759F03 Chr1:11182449
   
Insertion allele amplicon 3144 - FQ35
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer FQ35: 5'-CTGAAAATGTTCTTCTGACCATCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:11182713
   
Insertion allele amplicon 8474 - HM29
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer HM29: 5'-AGTAGGAGGAGTCGCTGGTAGAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:11182091
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11