Genotyping and primer details


Genotyping and primer details for 399G07 - At1g32850
Line ID 399G07
Locus At1g32850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:11906832 of line 399G07 Chr1:11906832
   
Insertion allele amplicon 8474 - CB54
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CB54: 5'-AAGAAGACAATGCAAGAGGGAATA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:11906295
   
wt amplicon (over InsPos) 07dd - CB54
wt amplicon start Chr1:11906295
wt amplicon end Chr1:11907220
wt amplicon size 926 bp
Locus specific primer CB54: 5'-AAGAAGACAATGCAAGAGGGAATA-3'
2nd locus specific primer 07dd: 5'-TTGAGCTATTTTCACGGCTATTTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11