Genotyping and primer details


Genotyping and primer details for 699A06 - At1g48480
Line ID 699A06
Locus At1g48480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:17919486 of line 699A06 Chr1:17919486
   
Insertion allele amplicon 8474 - EU40
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer EU40: 5'-TGTTAAACACATTGTCCCTACCAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:17920053
   
wt amplicon (over InsPos) TK47 - EU40
wt amplicon start Chr1:17918952
wt amplicon end Chr1:17920053
wt amplicon size 1102 bp
Locus specific primer EU40: 5'-TGTTAAACACATTGTCCCTACCAA-3'
2nd locus specific primer TK47: 5'-GAAATCTCTTCAGGTTTCACGAAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11