Genotyping and primer details


Genotyping and primer details for 834B05 - At1g48840
Line ID 834B05
Locus At1g48840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:18063062 of line 834B05 Chr1:18063062
   
Insertion allele amplicon 8474 - 60us
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 60us: 5'-CTGCTAGCCAGACATTACTCAGTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:18062493
   
Insertion allele amplicon 3144 - 67wa
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 67wa: 5'-GTGCAATGTCCCTGATAAAAGAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:18063754
   
wt amplicon (over InsPos) 67wa - 60us
wt amplicon start Chr1:18062493
wt amplicon end Chr1:18063754
wt amplicon size 1262 bp
Locus specific primer 60us: 5'-CTGCTAGCCAGACATTACTCAGTG-3'
2nd locus specific primer 67wa: 5'-GTGCAATGTCCCTGATAAAAGAAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11