Genotyping and primer details


Genotyping and primer details for 175G01 - At1g53420
Line ID 175G01
Locus At1g53420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:19927163 of line 175G01 Chr1:19927163
   
Insertion allele amplicon 8474 - MN53
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MN53: 5'-TCTCTTGCCCTATCATTTCGTAGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:19926685
   
Insertion allele amplicon 8474 - NH57
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer NH57: 5'-TTATTTGATGGAACAATAATCGCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:19927818
   
wt amplicon (over InsPos) MN53 - NH57
wt amplicon start Chr1:19926685
wt amplicon end Chr1:19927818
wt amplicon size 1134 bp
Locus specific primer NH57: 5'-TTATTTGATGGAACAATAATCGCA-3'
2nd locus specific primer MN53: 5'-TCTCTTGCCCTATCATTTCGTAGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11