Genotyping and primer details


Genotyping and primer details for 529E10 - At1g55610
Line ID 529E10
Locus At1g55610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:20783271 of line 529E10 Chr1:20783271
   
Insertion allele amplicon 8474 - EQ91
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer EQ91: 5'-AAGAAGGTGAGATTCCCACAGATA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:20782683
   
wt amplicon (over InsPos) KR77 - EQ91
wt amplicon start Chr1:20782683
wt amplicon end Chr1:20783823
wt amplicon size 1141 bp
Locus specific primer EQ91: 5'-AAGAAGGTGAGATTCCCACAGATA-3'
2nd locus specific primer KR77: 5'-TTAAACCATCTGATTTTGGAAAGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11