Genotyping and primer details


Genotyping and primer details for 323B01 - At1g56140
Line ID 323B01
Locus At1g56140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:21002149 of line 323B01 Chr1:21002149
   
Insertion allele amplicon 8474 - EQ22
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer EQ22: 5'-TACACTGTAAAAGAGACGGCATTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:21001492
   
Insertion allele amplicon 8474 - KH55
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KH55: 5'-GCAAAACTGTACGATGACAAGAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:20995687
   
wt amplicon (over InsPos) KH55 - EQ22
wt amplicon start Chr1:20995687
wt amplicon end Chr1:21001492
wt amplicon size 1012 bp
Locus specific primer EQ22: 5'-TACACTGTAAAAGAGACGGCATTG-3'
2nd locus specific primer KH55: 5'-GCAAAACTGTACGATGACAAGAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11