Genotyping and primer details


Genotyping and primer details for 342G08 - At1g61630
Line ID 342G08
Locus At1g61630 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:22744347 of line 342G08 Chr1:22744347
   
Insertion allele amplicon 8474 - 04ih
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 04ih: 5'-TACTCGAAAATTGTGAAGAACGTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:22744025
   
wt amplicon (over InsPos) 11ji - 04ih
wt amplicon start Chr1:22744025
wt amplicon end Chr1:22744783
wt amplicon size 759 bp
Locus specific primer 04ih: 5'-TACTCGAAAATTGTGAAGAACGTG-3'
2nd locus specific primer 11ji: 5'-ACCGAATCAAATCAAGTCAAAATC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11